PUBLICATION
Synergistic effect of fenpropathrin and paclobutrazol on early life stages of zebrafish (Danio rerio)
- Authors
- Wang, Y., Yang, G., Shen, W., Xu, C., Di, S., Wang, D., Li, X., Wang, X., Wang, Q.
- ID
- ZDB-PUB-200708-15
- Date
- 2020
- Source
- Environmental pollution (Barking, Essex : 1987) 266: 115067 (Journal)
- Registered Authors
- Keywords
- Biomarker, Combined toxicity, Embryonic zebrafish, Toxicity mechanism
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Larva
- Pyrethrins
- Triazoles
- Water Pollutants, Chemical*
- Zebrafish*
- PubMed
- 32629307 Full text @ Environ. Pollut.
Citation
Wang, Y., Yang, G., Shen, W., Xu, C., Di, S., Wang, D., Li, X., Wang, X., Wang, Q. (2020) Synergistic effect of fenpropathrin and paclobutrazol on early life stages of zebrafish (Danio rerio). Environmental pollution (Barking, Essex : 1987). 266:115067.
Abstract
Aquatic organisms are usually exposed to various co-existing pollutants. However, toxic effects of pesticide mixtures on aquatic organisms and its potential underlying mechanism still remain unclear. The joint effects of fenpropathrin (FEN) and paclobutrazol (PAC) on zebrafish (Danio rerio) using diverse toxicological endpoints were investigated in the current work. Our data exhibited that the 96-h LC50 values of FEN to zebrafish at multiple life phases ranged from 0.0029 (0.0013-0.0042) to 0.16 (0.082-0.23) mg a.i. L-1, which were lower by comparison to PAC ranging from 13.16 (8.564-21.03) to 23.43 (17.94-29.91) mg a.i. L-1. Combination of FEN and PAC displayed synergistic effect on embryonic zebrafish. Activities of T-SOD, Cu/Zn-SOD and CYP450 were remarkably changed in the majority of single and mixture treatments by comparison to the untreated group. The mRNA levels of 17 genes related to oxidative stress, cellular apoptosis, immune system and endocrine system were assessed, and the data suggested that embryonic zebrafish were affected by both single pesticides and their mixtures. Five genes (P53, tsh, ERα, crh and cxcl-clc) showed greater alterations when exposed to pesticide mixtures by comparison to their individual chemicals. Therefore, it is urgently necessary to conduct more studies on mixture toxicities of different pesticides to explore the chemical mixtures with synergistic interactions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping