PUBLICATION
A far-red fluorescent chemogenetic reporter for in vivo molecular imaging
- Authors
- Li, C., Tebo, A.G., Thauvin, M., Plamont, M.A., Volovitch, M., Morin, X., Vriz, S., Gautier, A.
- ID
- ZDB-PUB-200623-4
- Date
- 2020
- Source
- Angewandte Chemie (International ed. in English) 59(41): 17917-17923 (Journal)
- Registered Authors
- Vriz, Sophie
- Keywords
- far-red fluorescence, fluorescent chemogenetic reporter, fluorogenic chromophore, in vivo fluorescence imaging
- MeSH Terms
-
- Fluorescent Dyes/chemistry*
- Animals
- Zebrafish/embryology
- Molecular Imaging/methods*
- Chick Embryo
- HeLa Cells
- Protein Binding
- Humans
- PubMed
- 32568417 Full text @ Angew. Chem. Int. Ed. Engl.
Citation
Li, C., Tebo, A.G., Thauvin, M., Plamont, M.A., Volovitch, M., Morin, X., Vriz, S., Gautier, A. (2020) A far-red fluorescent chemogenetic reporter for in vivo molecular imaging. Angewandte Chemie (International ed. in English). 59(41):17917-17923.
Abstract
Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non- fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae and chicken embryo. Beyond enabling genetic encoding of far-red fluorescence, frFAST allowed the design of a far- red chemogenetic reporter of protein-protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping