ZFIN ID: ZDB-PUB-200623-4
A far-red fluorescent chemogenetic reporter for in vivo molecular imaging
Li, C., Tebo, A.G., Thauvin, M., Plamont, M.A., Volovitch, M., Morin, X., Vriz, S., Gautier, A.
Date: 2020
Source: Angewandte Chemie (International ed. in English)   59(41): 17917-17923 (Journal)
Registered Authors: Vriz, Sophie
Keywords: far-red fluorescence, fluorescent chemogenetic reporter, fluorogenic chromophore, in vivo fluorescence imaging
MeSH Terms:
  • Animals
  • Chick Embryo
  • Fluorescent Dyes/chemistry*
  • HeLa Cells
  • Humans
  • Molecular Imaging/methods*
  • Protein Binding
  • Zebrafish/embryology
PubMed: 32568417 Full text @ Angew. Chem. Int. Ed. Engl.
ABSTRACT
Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non- fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae and chicken embryo. Beyond enabling genetic encoding of far-red fluorescence, frFAST allowed the design of a far- red chemogenetic reporter of protein-protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.
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