ZFIN ID: ZDB-PUB-200618-6
NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish
Mattern, K., Trotha, J.W.V., Erfle, P., Köster, R.W., Dietzel, A.
Date: 2020
Source: Communications biology   3: 311 (Journal)
Registered Authors: Köster, Reinhard W., von Trotha, Jakob
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Brain/diagnostic imaging*
  • Calcium Signaling
  • Computer-Aided Design
  • Equipment Design
  • Glass
  • Hydrodynamics
  • Lab-On-A-Chip Devices*
  • Larva
  • Microfluidics/instrumentation
  • Microscopy/instrumentation*
  • Microscopy/methods
  • Zebrafish*/genetics
PubMed: 32546816 Full text @ Commun Biol
While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, however, have not been combined to monitor whole-brain neural activity yet. Unlike conventional microfluidics, we report here an all-glass device (NeuroExaminer) that is compatible with whole-brain in vivo imaging using light-sheet microscopy and can thus provide insights into brain function in health and disease.