PUBLICATION
Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio)
- Authors
- Wu, L., Chen, H., Ru, H., Li, Y., Yao, F., Ni, Z., Zhong, L.
- ID
- ZDB-PUB-200612-11
- Date
- 2020
- Source
- Toxicology letters 331: 143-151 (Journal)
- Registered Authors
- Keywords
- Hypothalamus-pituitary-thyroid-liver (HPTL) axis, TPT, Thyroid disruption, metabolizing enzymes
- MeSH Terms
-
- Animals
- Brain/drug effects
- Brain/enzymology
- Dose-Response Relationship, Drug
- Endocrine Disruptors/toxicity*
- Female
- Gene Expression/drug effects
- Liver/drug effects
- Liver/enzymology
- Male
- Organotin Compounds/toxicity*
- Sex Characteristics*
- Thyroid Gland/drug effects*
- Thyroid Gland/metabolism
- Thyroid Hormones/genetics
- Thyroid Hormones/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism*
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 32525014 Full text @ Toxicol. Lett.
- CTD
- 32525014
Citation
Wu, L., Chen, H., Ru, H., Li, Y., Yao, F., Ni, Z., Zhong, L. (2020) Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio). Toxicology letters. 331:143-151.
Abstract
Although organotin compounds are known to disturb thyroid signaling and antioxidant defense system, the sex-differences underlying these effects of triphenyltin chloride (TPT) in fish remain unclear. To understand these differences, adult zebrafish (Danio rerio) were exposed to different concentrations of TPT (0, 10, 100, or 1000 ng/L) for 28 days. Female zebrafish exposed to TPT showed significantly increased thyroxine (T4) content and decrease triiodothyronine (T3) content, possibly due to downregulation of deiodinase (dio2) and uridine diphosphate glucuronosyl transferase (ugt1ab). However, decreased T4 and T3 contents in male zebrafish accompanied with upregulation of dio1, dio2 and ugt1ab. TPT exposure can lead to sex-specific thyroid disruption in adult zebrafish via alterations the Hypothalamus-pituitary-thyroid-liver axis. In addition, the gene expression levels of metabolizing enzymes, such as cyp1b, cyp1c, gpx1a, or sult1st1 were also to vary in a sex-dependent manner in adult zebrafish liver. Downregulation of cyp19a and cyp19b and decreased 17β-estradiol (E2) contents were detected in both female and male zebrafish. Therefore, a sex-specific of thyroid disruption response after TPT exposure was observed in adult zebrafish, possibly due to inherent in female or males detoxifying enzyme capacities.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping