ZFIN ID: ZDB-PUB-200606-5
Single-cell transcriptomic analysis identifies the conversion of zebrafish Etv2-deficient vascular progenitors into skeletal muscle
Chestnut, B., Casie Chetty, S., Koenig, A.L., Sumanas, S.
Date: 2020
Source: Nature communications   11: 2796 (Journal)
Registered Authors: Sumanas, Saulius
Keywords: none
Microarrays: GEO:GSE142484
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Biomarkers/metabolism
  • Blood Vessels/cytology*
  • Cell Differentiation/genetics
  • Cell Movement
  • Embryo, Nonmammalian/metabolism
  • Fibroblast Growth Factors/metabolism
  • Gene Expression Profiling*
  • Green Fluorescent Proteins/metabolism
  • Heat-Shock Response
  • Models, Biological
  • Muscle, Skeletal/cytology*
  • Mutation/genetics
  • Single-Cell Analysis*
  • Somites/metabolism
  • Stem Cells/metabolism*
  • Transcription, Genetic
  • Wnt Signaling Pathway
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/deficiency*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 32493965 Full text @ Nat. Commun.
Cell fate decisions involved in vascular and hematopoietic embryonic development are still poorly understood. An ETS transcription factor Etv2 functions as an evolutionarily conserved master regulator of vasculogenesis. Here we report a single-cell transcriptomic analysis of hematovascular development in wild-type and etv2 mutant zebrafish embryos. Distinct transcriptional signatures of different types of hematopoietic and vascular progenitors are identified using an etv2ci32Gt gene trap line, in which the Gal4 transcriptional activator is integrated into the etv2 gene locus. We observe a cell population with a skeletal muscle signature in etv2-deficient embryos. We demonstrate that multiple etv2ci32Gt; UAS:GFP cells differentiate as skeletal muscle cells instead of contributing to vasculature in etv2-deficient embryos. Wnt and FGF signaling promote the differentiation of these putative multipotent etv2 progenitor cells into skeletal muscle cells. We conclude that etv2 actively represses muscle differentiation in vascular progenitors, thus restricting these cells to a vascular endothelial fate.