PUBLICATION

Expression of a Barhl1a reporter in subsets of retinal ganglion cells and commissural neurons of the developing zebrafish brain

Authors
Albadri, S., Armant, O., Aljand-Geschwill, T., Del Bene, F., Carl, M., Strähle, U., Poggi, L.
ID
ZDB-PUB-200603-13
Date
2020
Source
Scientific Reports   10: 8814 (Journal)
Registered Authors
Albadri, Shahad, Armant, Olivier, Carl, Matthias, Del Bene, Filippo, Poggi, Lucia, Strähle, Uwe
Keywords
none
MeSH Terms
  • Amacrine Cells/metabolism
  • Animals
  • Axons/ultrastructure
  • Cell Lineage
  • DNA-Binding Proteins/physiology
  • Diencephalon/cytology
  • Diencephalon/embryology
  • Gene Duplication
  • Gene Expression Regulation, Developmental*
  • Genes, Reporter
  • Homeodomain Proteins/biosynthesis*
  • Homeodomain Proteins/genetics
  • Intravital Microscopy
  • Microscopy, Fluorescence
  • Nerve Tissue Proteins/biosynthesis*
  • Neural Stem Cells/metabolism
  • Neurons/metabolism
  • Optic Chiasm/cytology
  • Optic Chiasm/embryology*
  • Retinal Ganglion Cells/classification
  • Retinal Ganglion Cells/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology
PubMed
32483163 Full text @ Sci. Rep.
Abstract
Promoting the regeneration or survival of retinal ganglion cells (RGCs) is one focus of regenerative medicine. Homeobox Barhl transcription factors might be instrumental in these processes. In mammals, only barhl2 is expressed in the retina and is required for both subtype identity acquisition of amacrine cells and for the survival of RGCs downstream of Atoh7, a transcription factor necessary for RGC genesis. The underlying mechanisms of this dual role of Barhl2 in mammals have remained elusive. Whole genome duplication in the teleost lineage generated the barhl1a and barhl2 paralogues. In the Zebrafish retina, Barhl2 functions as a determinant of subsets of amacrine cells lineally related to RGCs independently of Atoh7. In contrast, barhl1a expression depends on Atoh7 but its expression dynamics and function have not been studied. Here we describe for the first time a Barhl1a reporter line in vivo showing that barhl1a turns on exclusively in subsets of RGCs and their post-mitotic precursors. We also show transient expression of barhl1a:GFP in diencephalic neurons extending their axonal projections as part of the post-optic commissure, at the time of optic chiasm formation. This work sets the ground for future studies on RGC subtype identity, axonal projections and genetic specification of Barhl1a-positive RGCs and commissural neurons.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes