PUBLICATION
Effects of lincomycin hydrochloride on the neurotoxicity of zebrafish
- Authors
- Cheng, B., Jiang, F., Su, M., Zhou, L., Zhang, H., Cao, Z., Liao, X., Xiong, G., Xiao, J., Liu, F., Lu, H.
- ID
- ZDB-PUB-200601-14
- Date
- 2020
- Source
- Ecotoxicology and environmental safety 201: 110725 (Journal)
- Registered Authors
- Lu, Huiqiang
- Keywords
- Developmental toxicity, Lincomycin hydrochloride, Neurotoxicity, Oxidative stress, Zebrafish
- MeSH Terms
-
- Acetylcholinesterase/metabolism
- Adenosine Triphosphatases/metabolism
- Animals
- Apoptosis/drug effects
- Embryonic Development/drug effects
- Larva/drug effects
- Larva/growth & development
- Lincomycin/toxicity*
- Neurotoxicity Syndromes/congenital
- Neurotoxicity Syndromes/etiology*
- Oxidative Stress/drug effects
- Zebrafish
- PubMed
- 32474209 Full text @ Ecotoxicol. Environ. Saf.
Citation
Cheng, B., Jiang, F., Su, M., Zhou, L., Zhang, H., Cao, Z., Liao, X., Xiong, G., Xiao, J., Liu, F., Lu, H. (2020) Effects of lincomycin hydrochloride on the neurotoxicity of zebrafish. Ecotoxicology and environmental safety. 201:110725.
Abstract
Lincomycin hydrochloride is one of the commonly used drugs in clinic. However, it has many side effects on patients, and its mechanism is still poorly understood. In this study, 6 h post-fertilization (6 hpf) zebrafish embryos were exposed to several concentrations of lincomycin hydrochloride (15, 30, 60 μg/mL) for up to 24 or 96 hpf to detect their developmental toxicity and neurotoxicity, and to 6 days post-fertilization (6 dpf) to detect their behavioral toxicity. Our results showed that lincomycin hydrochloride could lead to embryonic head deformities (unclear ventricles, smaller ventricles, fewer new neurons). The studies showed that the frequency of spontaneous tail flick of zebrafish embryo increased at 24 hpf, and the lincomycin hydrochloride exposed zebrafish embryos showed increased heart rate, shorter body length, and yolk sac edema with severe pericardial edema at 96 hpf. The studies also showed that lincomycin hydrochloride increased oxidative stress level, Acetylcholinesterase (AChE) activity, ATPase activity and apoptosis in zebrafish larvae. In addition, the swimming behavior of zebrafish larvae decreased with the increase of lincomycin hydrochloride concentration, but the angular velocity and meandering degree increased, which might be due to the decreased activity of AChE and ATPase, as well as the decreased expression of genes related to neurodevelopment and neurotransmitter system, leading to the change of their motor behaviors. In summary, we found that lincomycin hydrochloride induced developmental toxicity and neurotoxicity in zebrafish larvae, contributing to a more comprehensive evaluation of the safety of the drug.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping