ZFIN ID: ZDB-PUB-200530-7
Unifying Developmental Programs for Embryonic and Post-Embryonic Neurogenesis in the Zebrafish Retina
Xu, B., Tang, X., Jin, M., Zhang, H., Du, L., Yu, S., He, J.
Date: 2020
Source: Development (Cambridge, England)   147(12): (Journal)
Registered Authors: Du, Lei, He, Jie, Jin, Mengmeng, Tang, Xia, Xu, Baijie, Yu, Shuguang, Zhang, Hui
Keywords: Developmental state, Retinal progenitor cells, Retinal stem cells, Single-cell RNA sequencing, Zebrafish
Microarrays: GEO:GSE122680
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Embryo, Nonmammalian/metabolism*
  • Embryonic Development
  • Fatty Acid-Binding Proteins/deficiency
  • Fatty Acid-Binding Proteins/genetics
  • Fatty Acid-Binding Proteins/metabolism
  • Gene Expression Regulation, Developmental
  • Neurogenesis/genetics*
  • Retina/cytology
  • Retina/growth & development
  • Retina/metabolism*
  • Sequence Analysis, RNA
  • Single-Cell Analysis
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 32467236 Full text @ Development
The zebrafish retina grows for a lifetime. Whether embryonic and post-embryonic retinogenesis conform to the same developmental program is an outstanding question that remains under debate. Using single-cell RNA sequencing of ∼20,000 cells of the developing zebrafish retina at four different stages, we identified seven distinct developmental states. Each state explicitly expresses a gene set. Disruption of individual state-specific marker genes results in various defects ranging from small eyes to the loss of distinct retinal cell types. Using a similar approach, we further characterized the developmental states of post-embryonic retinal stem cells (RSCs) and their progeny in the ciliary marginal zone. Expression pattern analysis of state-specific marker genes showed that the developmental states of post-embryonic RSCs largely recapitulated those of their embryonic counterparts, except for some differences in rod photoreceptor genesis. Thus, our findings reveal the unifying developmental program used by the embryonic and post-embryonic retinogenesis in zebrafish.