ZFIN ID: ZDB-PUB-200529-13
Optical control of protein activity and gene expression by photoactivation of caged cyclofen
Hamouri, F., Zhang, W., Aujard, I., Le Saux, T., Ducos, B., Vriz, S., Jullien, L., Bensimon, D.
Date: 2019
Source: Methods in enzymology   624: 1-23 (Journal)
Registered Authors: Bensimon, David, Ducos, Bertrand, Hamouri, Fatima, Vriz, Sophie, Zhang, Weiting
Keywords: Cancer, Development, Optogenetics, Photoactivation
MeSH Terms:
  • Animals
  • Gene Expression
  • Light
  • Optogenetics/methods*
  • Photochemical Processes
  • Polycyclic Compounds/chemistry*
  • Receptors, Estrogen/chemistry
  • Receptors, Estrogen/genetics*
  • Transcriptional Activation*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed: 31370925 Full text @ Methods Enzymol.
ABSTRACT
The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. While many of these approaches use a fusion between a light activatable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly and locally in a live organism. Here, we present the experimental details behind this approach.
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