|ZFIN ID: ZDB-PUB-200529-13|
Optical control of protein activity and gene expression by photoactivation of caged cyclofen
Hamouri, F., Zhang, W., Aujard, I., Le Saux, T., Ducos, B., Vriz, S., Jullien, L., Bensimon, D.
|Source:||Methods in enzymology 624: 1-23 (Chapter)|
|Registered Authors:||Bensimon, David, Ducos, Bertrand, Hamouri, Fatima, Vriz, Sophie, Zhang, Weiting|
|Keywords:||Cancer, Development, Optogenetics, Photoactivation|
|PubMed:||31370925 Full text @ Methods Enzymol.|
Hamouri, F., Zhang, W., Aujard, I., Le Saux, T., Ducos, B., Vriz, S., Jullien, L., Bensimon, D. (2019) Optical control of protein activity and gene expression by photoactivation of caged cyclofen. Methods in enzymology. 624:1-23.
ABSTRACTThe use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. While many of these approaches use a fusion between a light activatable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly and locally in a live organism. Here, we present the experimental details behind this approach.
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