PUBLICATION
Fluoride impairs ovary development by affecting oogenesis and inducing oxidative stress and apoptosis in female zebrafish (Danio rerio)
- Authors
- Li, M., Cao, J., Zhao, Y., Wu, P., Li, X., Khodaei, F., Han, Y., Wang, J.
- ID
- ZDB-PUB-200526-18
- Date
- 2020
- Source
- Chemosphere 256: 127105 (Journal)
- Registered Authors
- Keywords
- Apoptosis, Female zebrafish, Fluoride, Oogenesis, Oxidative damage
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Apoptosis/drug effects
- Female
- Fluorides/toxicity*
- Oogenesis/drug effects
- Ovary/drug effects
- Oxidative Stress/drug effects
- Sex Differentiation
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism
- PubMed
- 32450357 Full text @ Chemosphere
Citation
Li, M., Cao, J., Zhao, Y., Wu, P., Li, X., Khodaei, F., Han, Y., Wang, J. (2020) Fluoride impairs ovary development by affecting oogenesis and inducing oxidative stress and apoptosis in female zebrafish (Danio rerio). Chemosphere. 256:127105.
Abstract
Previous studies have shown that waterborne fluoride exposure has adverse effects on the reproductive system of zebrafish. However, the underlying toxic mechanisms were still not clear. In the present study, female zebrafish were exposed to different concentrations of 0.787 (Control), 18.599, 36.832 mg/L of fluoride for 30 d and 60 d, and the effects of different doses of fluoride on ovary development, reproductive hormones, oogenesis, ROS content, antioxidant levels, and the expression of apoptosis-related genes and proteins in the ovaries of female zebrafish were analyzed. The results showed that ovarian weight and GSI were significantly decreased, FSH, LH and VTG levels were significantly reduced, the transcriptional profiles of oogenesis-related genes (tgfβ1, bmp15, gdf9, mprα, mprβ, ptg2β) were remarkably altered, ROS levels was notably increased, the SOD, CAT, GPx activities and GSH content as well as their mRNA expressions were significantly decreased, MDA content was remarkably increased, the expressions of apoptosis-related genes and proteins (caspase3, caspase8, caspase9, Fas-L, Cytochrome C, Bax and Bcl-2) were significantly changed, the ratio of Bax/Bcl-2 protein levels were notably increased. Taken together, this study demonstrated that fluoride exposure significantly affected ovarian development, decreased the reproductive hormones, affected oogenesis, induced oxidative stress, caused apoptosis through both extrinsic and intrinsic pathways in ovary of zebrafish. Indicating that oogenesis, oxidative stress, and apoptosis were responsible for the impairment of ovarian development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping