PUBLICATION
Pyclen based Ln(III) complexes as highly luminescent bioprobes for in vitro and in vivo one- and two-photon bioimaging applications
- Authors
- Hamon, N., Roux, A., Beyler, M., Mulatier, J.C., Andraud, C., Nguyen, C., Maynadier, M., Bettache, N., Duperray, A., Grichine, A., Brasselet, S., Gary-Bobo, M., Maury, O., Tripier, R.
- ID
- ZDB-PUB-200506-15
- Date
- 2020
- Source
- Journal of the American Chemical Society 142(22): 10184-10197 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Azabicyclo Compounds/chemistry*
- Coordination Complexes/chemical synthesis
- Coordination Complexes/chemistry*
- Humans
- Lanthanoid Series Elements/chemistry*
- Ligands
- Luminescent Agents/chemical synthesis
- Luminescent Agents/chemistry*
- MCF-7 Cells
- Molecular Structure
- Optical Imaging*
- Photons*
- Zebrafish/embryology
- PubMed
- 32368907 Full text @ J. Am. Chem. Soc.
Citation
Hamon, N., Roux, A., Beyler, M., Mulatier, J.C., Andraud, C., Nguyen, C., Maynadier, M., Bettache, N., Duperray, A., Grichine, A., Brasselet, S., Gary-Bobo, M., Maury, O., Tripier, R. (2020) Pyclen based Ln(III) complexes as highly luminescent bioprobes for in vitro and in vivo one- and two-photon bioimaging applications. Journal of the American Chemical Society. 142(22):10184-10197.
Abstract
In addition to the already described ligand L4a, two pyclen based lanthanide chelators, L4b and L4c, bearing two specific picolinate two-photon antennas (tailor-made for each targeted metal) and one acetate arm arranged in a dissymmetrical manner, have been synthesized, to form a complete family of lanthanide luminescent bioprobes: [EuL4a], [SmL4a], [YbL4b], [TbL4c] and [DyL4c]. Additionally, symmetrically arranged regioisomer L4a' was also synthesized as well as its [EuL4a'] complex to highlight the aston-ishing positive impact of the dissymmetrical N-distribution of the functional chelating arm. The investigation clearly shows the high performance of each bioprobe, which, depending on the complexed lanthanide, could be used in various applications. Each presents high brightness, quantum yields and lifetimes. Staining of the complexes into living human breast cancer cells was ob-served. In addition, in vivo two-photon microscopy was performed for the first time on a living Zebrafish model with [EuL4a]. No apparent toxicity was detected on the growth of the zebrafish and images of high quality were obtained.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping