PUBLICATION
Persistent Exposure to Environmental Levels of Microcystin-LR Disturbs Cortisol Production via Hypothalamic-Pituitary-Interrenal (HPI) Axis and Subsequently Liver Glucose Metabolism in Adult Male Zebrafish (Danio rerio)
- Authors
- Wang, L., Lin, W., Zha, Q., Guo, H., Zhang, D., Yang, L., Li, L., Li, D., Tang, R.
- ID
- ZDB-PUB-200502-1
- Date
- 2020
- Source
- toxins 12(5): (Journal)
- Registered Authors
- Keywords
- cortisol, hypothalamic-pituitary-interrenal axis, liver glucose metabolism, microcystin-LR, zebrafish
- MeSH Terms
-
- Animals
- Blood Glucose/drug effects*
- Blood Glucose/metabolism
- Endocrine Disruptors/toxicity*
- Gene Expression Regulation
- Homeostasis
- Hydrocortisone/blood*
- Hypothalamo-Hypophyseal System/drug effects*
- Hypothalamo-Hypophyseal System/metabolism
- Kidney/drug effects*
- Kidney/metabolism
- Liver/drug effects*
- Liver/metabolism
- Male
- Marine Toxins/toxicity*
- Microcystins/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32353954 Full text @ Toxins (Basel)
Citation
Wang, L., Lin, W., Zha, Q., Guo, H., Zhang, D., Yang, L., Li, L., Li, D., Tang, R. (2020) Persistent Exposure to Environmental Levels of Microcystin-LR Disturbs Cortisol Production via Hypothalamic-Pituitary-Interrenal (HPI) Axis and Subsequently Liver Glucose Metabolism in Adult Male Zebrafish (Danio rerio). toxins. 12(5):.
Abstract
There is growing evidence that microcystin-LR (MC-LR) is a new endocrine disruptor, whereas the impacts of persistent exposure to MC-LR on the hypothalamic-pituitary-interrenal (HPI) axis and health hazards thereafter have not been investigated. In this work, adult male zebrafish (Danio rerio) were immersed into MC-LR solutions at concentrations of 0, 1, 5 and 25 μg/L for 30 d, respectively. The results showed that persistent MC-LR exposure caused an extensive upregulation of HPI-axis genes but an inhibition of brain nuclear receptors (gr and mr), which finally increased serum cortisol levels. Furthermore, the decreased expression of hepatic gr might partly be responsible for the strong inhibition on the expression of downstream genes involved in glucose metabolic enzymes, including gluconeogenesis-related genes (pepck, fbp1a, g6pca), glycogenolysis-related gene (pyg), glycolysis-related genes (gk, pfk1b, pk) and glycogenesis-related gene (gys2). These findings are in accordance with the decline in serum glucose, indicating that long-term MC-LR exposure caused a lower production of glucose relative to glucose lysis. Our above results firstly establish the link between persistent MC-LR exposure and impaired glucose metabolism, suggesting that long-term MC-LR-mediated stress might threaten fish's health.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping