PUBLICATION
Highlights on selected microscopy techniques to study zebrafish developmental biology
- Authors
- Abu-Siniyeh, A., Al-Zyoud, W.
- ID
- ZDB-PUB-200430-7
- Date
- 2020
- Source
- Laboratory animal research 36: 12 (Review)
- Registered Authors
- Abu-Siniyeh, Ahmed
- Keywords
- Animal model, Development, Light-Sheet Microscopy, Second Harmonic Generation, Two-Photon Microscopy, Zebrafish
- MeSH Terms
- none
- PubMed
- 32346532 Full text @ Lab Anim Res
Citation
Abu-Siniyeh, A., Al-Zyoud, W. (2020) Highlights on selected microscopy techniques to study zebrafish developmental biology. Laboratory animal research. 36:12.
Abstract
Bio-imaging is a tedious task when it concerns exploring cell functions, developmental mechanisms, and other vital processes in vivo. Single-cell resolution is challenging due to different issues such as sample size, the scattering of intact and opaque tissue, pigmentation in untreated animals, the movement of living organs, and maintaining the sample under physiological conditions. These factors might lead researchers to implement microscopy techniques with a suitable animal model to mimic the nature of the living cells. Zebrafish acquired its prestigious reputation in the biomedical research field due to its transparency under advanced microscopes. Therefore, various microscopy techniques, including Multi-Photon, Light-Sheet Microscopy, and Second Harmonic Generation, simplify the discovery of different types of internal functions in zebrafish. In this review, we briefly discuss three recent microscopy techniques that are being utilized because they are non-invasive in investigating developmental events in zebrafish embryo and larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping