PUBLICATION
Trade-off between somatic and germline repair in a vertebrate supports the expensive germ line hypothesis
- Authors
- Chen, H.Y., Jolly, C., Bublys, K., Marcu, D., Immler, S.
- ID
- ZDB-PUB-200422-21
- Date
- 2020
- Source
- Proceedings of the National Academy of Sciences of the United States of America 117(16): 8973-8979 (Journal)
- Registered Authors
- Keywords
- aging, germ line, somatic maintenance
- MeSH Terms
-
- Aging/physiology*
- Animals
- Animals, Genetically Modified
- Embryo, Nonmammalian/radiation effects
- Female
- Gene Knockdown Techniques
- Germ Cells/physiology
- Germ Cells/radiation effects
- Male
- Models, Animal
- RNA-Binding Proteins/genetics
- Regeneration/physiology*
- Sex Factors
- Stress, Physiological*
- Whole-Body Irradiation
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 32245815 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Chen, H.Y., Jolly, C., Bublys, K., Marcu, D., Immler, S. (2020) Trade-off between somatic and germline repair in a vertebrate supports the expensive germ line hypothesis. Proceedings of the National Academy of Sciences of the United States of America. 117(16):8973-8979.
Abstract
The disposable soma theory is a central tenet of the biology of aging where germline immortality comes at the cost of an aging soma [T. B. L. Kirkwood, Nature 270, 301-304 (1977); T. B. L. Kirkwood, Proc. R. Soc. Lond. B Biol. Sci. 205, 531-546 (1979); T. B. L. Kirkwood, S. N. Austad, Nature 408, 233-238 (2000)]. Limited resources and a possible trade-off between the repair and maintenance of the germ cells and growth and maintenance of the soma may explain the deterioration of the soma over time. Here we show that germline removal allows accelerated somatic healing under stress. We tested "the expensive germ line" hypothesis by generating germline-free zebrafish Danio rerio and testing the effect of the presence and absence of the germ line on somatic repair under benign and stressful conditions. We exposed male fish to sublethal low-dose ionizing radiation, a genotoxic stress affecting the soma and the germ line, and tested how fast the soma recovered following partial fin ablation. We found that somatic recovery from ablation occurred substantially faster in irradiated germline-free fish than in the control germline-carrying fish where somatic recovery was stunned. The germ line did show signs of postirradiation recovery in germline-carrying fish in several traits related to offspring number and fitness. These results support the theoretical conjecture that germline maintenance is costly and directly trades off with somatic maintenance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping