PUBLICATION

New minor groove covering DNA binding mode of dinuclear Pt(II) complexes with various pyridine-linked bridging ligands and dual anticancer-antiangiogenic activities

Authors
Franich, A.A., ?ivkovi?, M.D., Ili?-Tomi?, T., ?or?evi?, I.S., Nikodinovi?-Runi?, J., Pavi?, A., Janji?, G.V., Rajkovi?, S.
ID
ZDB-PUB-200403-41
Date
2020
Source
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry   25(3): 395-409 (Journal)
Registered Authors
Keywords
Dinuclear platinum(II) complexes, Dual anticancer and anti-angiogenic activity, Minor groove covering
MeSH Terms
  • Drug Screening Assays, Antitumor
  • Ligands
  • Molecular Structure
  • Binding Sites/drug effects
  • Angiogenesis Inhibitors/chemical synthesis
  • Angiogenesis Inhibitors/chemistry
  • Angiogenesis Inhibitors/pharmacology*
  • Cell Line, Tumor
  • Organoplatinum Compounds/chemical synthesis
  • Organoplatinum Compounds/chemistry
  • Organoplatinum Compounds/pharmacology*
  • Cell Proliferation/drug effects
  • Neoplasms, Experimental/drug therapy
  • Neoplasms, Experimental/metabolism
  • Neoplasms, Experimental/pathology
  • Pyridines/chemistry
  • Pyridines/pharmacology*
  • Molecular Docking Simulation
  • Dose-Response Relationship, Drug
  • Animals
  • Viscosity
  • Antineoplastic Agents/chemical synthesis
  • Antineoplastic Agents/chemistry
  • Antineoplastic Agents/pharmacology*
  • DNA/chemistry*
  • Humans
  • Zebrafish
  • Neovascularization, Pathologic/drug therapy*
  • Neovascularization, Pathologic/metabolism
  • Neovascularization, Pathologic/pathology
PubMed
32162071 Full text @ J. Biol. Inorg. Chem.
Abstract
New anticancer platinum(II) compounds simultaneously targeting tumor cells and tumor-derived neoangiogenesis, with new DNA interacting mode and large therapeutic window are appealing alternative to improve efficacy of clinical platinum chemotherapeutics. Herein, we describe three novel dinuclear [{Pt(en)Cl}2(μ-L)]2+ complexes with different pyridine-like bridging ligands (L), 4,4'-bipyridine (Pt1), 1,2-bis(4-pyridyl)ethane (Pt2) and 1,2-bis(4-pyridyl)ethene (Pt3), which highly, positively charged aqua derivatives, [{Pt(en)(H2O)}2(μ-L)]4+, interact with the phosphate backbone forming DNA-Pt adducts with an unique and previously undescribed binding mode, called a minor groove covering. The results of this study suggested that the new binding mode of the aqua-Pt(II) complexes with DNA could be attributed to the higher anticancer activities of their chloride analogues. All three compounds, particularly complex [{Pt(en)Cl}2(μ-4,4'-bipy)]Cl2·2H2O (4,4'-bipy is 4,4'-bipyridine) (Pt1), overcame cisplatin resistance in vivo in the zebrafish-mouse melanoma xenograft model, showed much higher therapeutic potential than antiangiogenic drug sunitinib malate, while effectively blocking tumor neovascularization and melanoma cell metastasis. Overall therapeutic profile showed new dinuclear Pt(II) complexes could be novel, effective and safe anticancer agents. Finally, the correlation with the structural characteristics of these complexes can serve as a useful tool for developing new and more effective anticancer drugs.
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