PUBLICATION
Effect of pterois volitans (lionfish) venom on cholinergic and dopaminergic systems
- Authors
- Becerra-Amezcua, M.P., Hernández-Sámano, A.C., Puch-Hau, C., Aguilar, M.B., Collí-Dulá, R.C.
- ID
- ZDB-PUB-200403-4
- Date
- 2020
- Source
- Environmental Toxicology and Pharmacology 77: 103359 (Journal)
- Registered Authors
- Keywords
- Acetylcholine, Acetylcholinesterase, Dopaminergic, Zebrafish, nAChR α2, nAChR α3β2
- MeSH Terms
-
- Acetylcholinesterase/genetics
- Animals
- Dopaminergic Neurons/drug effects
- Embryo, Nonmammalian
- Fish Proteins/physiology
- Fish Venoms/toxicity*
- Humans
- Oocytes/physiology
- Perciformes*
- Receptors, Nicotinic/physiology
- Xenopus laevis
- Zebrafish
- PubMed
- 32146351 Full text @ Environ. Toxicol. Pharmacol.
Citation
Becerra-Amezcua, M.P., Hernández-Sámano, A.C., Puch-Hau, C., Aguilar, M.B., Collí-Dulá, R.C. (2020) Effect of pterois volitans (lionfish) venom on cholinergic and dopaminergic systems. Environmental Toxicology and Pharmacology. 77:103359.
Abstract
Pterois volitans venom induces muscular fibrillation, which results from nerve transmission caused by the presence of acetylcholine (ACh). It also has cardiovascular effects that are due to its actions on muscarinic and nicotinic cholinergic receptors. In this study, we characterized the effects of P. volitans venom on nicotinic acetylcholine receptors (nAChRs) and dopaminergic neurons. After exposure to P. volitans venom, acetylcholinesterase (AChE) mRNA levels and the expression of the α2 subunit of nAChR increased in zebrafish embryos (15-20 somites). In addition, the lionfish venom blocked zebrafish α2 nAChR subunit functional expression and the ACh-induced response of human neuronal α3β2 receptors. The latter receptor was blocked by a protein fraction named F2, which was isolated from P. volitans venom using reversed phase high performance liquid chromatography (RP-HPLC). This venom causes death in dopaminergic neurons, and affects the cholinergic system. The effect of these two systems may result in retarded embryonic development of zebrafish, since the two systems function in a related manner to control growth hormone secretion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping