PUBLICATION
Isoliquiritin promote angiogenesis by recruiting macrophages to improve the healing of zebrafish wounds
- Authors
- Liu, Y.Y., Wu, J.Q., Fan, R.Y., He, Z.H., Li, C.Y., He, M.F.
- ID
- ZDB-PUB-200307-12
- Date
- 2020
- Source
- Fish & shellfish immunology 100: 238-245 (Journal)
- Registered Authors
- Keywords
- Angiogenesis, Isoliquiritin, Macrophage, Wound healing, Zebrafish
- MeSH Terms
-
- Animals
- Chalcone/analogs & derivatives*
- Chalcone/pharmacology
- Glucosides/pharmacology*
- Inflammation/genetics
- Macrophages/immunology*
- Neovascularization, Physiologic/drug effects*
- Plant Extracts/pharmacology*
- Plants, Medicinal/chemistry
- Skin/drug effects
- Skin/injuries
- Wound Healing/drug effects*
- Zebrafish/immunology*
- Zebrafish/injuries
- PubMed
- 32135341 Full text @ Fish Shellfish Immunol.
Citation
Liu, Y.Y., Wu, J.Q., Fan, R.Y., He, Z.H., Li, C.Y., He, M.F. (2020) Isoliquiritin promote angiogenesis by recruiting macrophages to improve the healing of zebrafish wounds. Fish & shellfish immunology. 100:238-245.
Abstract
Licorice is a widely used herbal medicine for the treatment of various diseases in southern Europe and parts of Asia. It has been reported that the isoliquiritin (ISL) from Glycyrrhiza root has the activity of promoting angiogenesis. The purpose of this study was to investigate the effect of ISL on the wound healing activity of zebrafish and its mechanism. 6-month-old zebrafish were injured in the skin (2 mm in diameter) and then treated with ISL. By measuring wound size and by histological examination, we found that ISL improved wound healing. In addition, 4-day-old zebrafish embryos of double transgenic line [Tg(fli-1:EGFP)]/[Tg(mpeg:mCherry)] were suffered from tissue traumas and then treated with ISL. Through fluorescent microscopy, we found that ISL promoted macrophage recruitment and angiogenesis in the wound area. Through qPCR analysis, we found that ISL up-regulated the expression of genes related to inflammation and angiogenesis in zebrafish embryos. These results showed that ISL could promote inflammatory response and angiogenesis, which played key roles in promoting wound healing. Therefore, ISL can be used as a promising candidate to promote wound healing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping