PUBLICATION
MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination
- Authors
- Liu, W., Jin, Y., Zhang, W., Xiang, Y., Jia, P., Yi, M., Jia, K.
- ID
- ZDB-PUB-200304-25
- Date
- 2020
- Source
- Viruses 12(3): (Journal)
- Registered Authors
- Keywords
- MiR-202-5p, RIG-I, TRIM25, red spotted grouper nervous necrosis virus, ubiquitination
- MeSH Terms
-
- Animals
- Fish Diseases/genetics*
- Fish Diseases/virology*
- Gene Expression Regulation*
- MicroRNAs/genetics
- Nodaviridae/physiology*
- RNA Interference*
- RNA Virus Infections/veterinary*
- Receptors, Immunologic/metabolism*
- Signal Transduction
- Ubiquitin-Protein Ligases/metabolism*
- Ubiquitination
- Zebrafish
- PubMed
- 32120903 Full text @ Viruses
Citation
Liu, W., Jin, Y., Zhang, W., Xiang, Y., Jia, P., Yi, M., Jia, K. (2020) MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination. Viruses. 12(3):.
Abstract
The RIG-I-like receptors (RLRs) signaling pathway is essential for inducing type I interferon (IFN) responses to viral infections. Meanwhile, it is also tightly regulated to prevent uncontrolled immune responses. Numerous studies have shown that microRNAs (miRNAs) are essential for the regulation of immune processes, however, the detailed molecular mechanism of miRNA regulating the RLRs signaling pathway remains to be elucidated. Here, our results showed that miR-202-5p was induced by red spotted grouper nervous necrosis virus (RGNNV) infection in zebrafish. Overexpression of miR-202-5p led to reduced expression of IFN 1 and its downstream antiviral genes, thus facilitating viral replication in vitro. In comparison, significantly enhanced levels of IFN 1 and antiviral genes and significantly low viral burden were observed in the miR-202-5p-/- zebrafish compared to wild type zebrafish. Subsequently, zebrafish tripartite motif-containing protein 25 (zbTRIM25) was identified as a target of miR-202-5p in both zebrafish and humans. Ectopic expression of miR-202-5p suppressed zbTRIM25-mediated RLRs signaling pathway. Furthermore, we showed that miR-202-5p inhibited zbTRIM25-mediated zbRIG-I ubiquitination and activation of IFN production. In conclusion, we demonstrate that RGNNV-inducible miR-202-5p acts as a negative regulator of zbRIG-I-triggered antiviral innate response by targeting zbTRIM25. Our study reveals a novel mechanism for the evasion of the innate immune response controlled by RGNNV.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping