PUBLICATION
Using zebrafish to assess the effect of chronic, early developmental exposure to environmentally relevant concentrations of 5-fluorouracil and leucovorin
- Authors
- Ng, M., DeCicco-Skinner, K., Connaughton, V.P.
- ID
- ZDB-PUB-200304-24
- Date
- 2020
- Source
- Environmental Toxicology and Pharmacology 76: 103356 (Journal)
- Registered Authors
- Connaughton, Victoria P.
- Keywords
- Behavior, Chemotherapy, Danio rerio, Growth, Survival
- MeSH Terms
-
- Animals
- Antidotes/toxicity*
- Antimetabolites/toxicity*
- Environmental Pollutants/chemistry*
- Fluorouracil/toxicity*
- Gene Expression Regulation, Developmental/drug effects
- Larva/drug effects
- Leucovorin/toxicity*
- Toxicity Tests
- Tumor Suppressor Protein p53/genetics
- Tumor Suppressor Protein p53/metabolism
- Zebrafish
- PubMed
- 32120338 Full text @ Environ. Toxicol. Pharmacol.
Citation
Ng, M., DeCicco-Skinner, K., Connaughton, V.P. (2020) Using zebrafish to assess the effect of chronic, early developmental exposure to environmentally relevant concentrations of 5-fluorouracil and leucovorin. Environmental Toxicology and Pharmacology. 76:103356.
Abstract
Environmental contaminants can deleteriously affect aquatic animals. One such contaminant is 5-fluorouracil (5-FU), a long-prescribed chemotherapeutic drug. Leucovorin (LV) is co-administered with 5-FU, potentiating its effects. Zebrafish (Danio rerio) larvae were reared in ng/L treatments of either 5-FU, LV, or a combined 5-FU/LV mixture for 8 dy. Survival was measured daily and swimming behavior assessed every other day. After 8 dy, larval length was measured, and densitometry of p53-labeled cryostat sections determined the extent of apoptosis. No significant differences in survival or apoptosis were found; larvae in the highest concentrations were largest. Changes in behavior of 5-FU-treated larvae were based on exposure duration; changes in LV-treated larvae were affected by drug concentration and duration. Larvae co-exposed to 5-FU/LV had responses like 5-FU-treated larvae. Overall, early developmental exposure of zebrafish larvae to environmentally-relevant concentrations of 5-FU and LV did not adversely affect survival, growth, and behavior suggesting realistic concentrations are sublethal and non-toxic.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping