PUBLICATION
Evaluation of the spinal effects of phthalates in a zebrafish embryo assay
- Authors
- Qian, L., Liu, J., Lin, Z., Chen, X., Yuan, L., Shen, G., Yang, W., Wang, D., Huang, Y., Pang, S., Mu, X., Wang, C., Li, Y.
- ID
- ZDB-PUB-200225-27
- Date
- 2020
- Source
- Chemosphere 249: 126144 (Journal)
- Registered Authors
- Keywords
- Behavioral effect, Phthalates, Spinal effects, Zebrafish embryo
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing
- Animals
- Biological Assay*
- Dibutyl Phthalate
- Diethylhexyl Phthalate
- Embryo, Nonmammalian/drug effects
- Larva
- Phthalic Acids/toxicity*
- Plasticizers
- Spine/drug effects*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- Zebrafish Proteins
- PubMed
- 32086060 Full text @ Chemosphere
Citation
Qian, L., Liu, J., Lin, Z., Chen, X., Yuan, L., Shen, G., Yang, W., Wang, D., Huang, Y., Pang, S., Mu, X., Wang, C., Li, Y. (2020) Evaluation of the spinal effects of phthalates in a zebrafish embryo assay. Chemosphere. 249:126144.
Abstract
Phthalates (phthalate esters, PAEs) are commonly used as plasticizers and are emerging concerns worldwide for their potential influence on the environment and general public health. Thus, identification of the negative effects and involved mechanisms of PAEs is necessary. Herein, we found that embryonic exposure of zebrafish to di-(2-ethylhexyl) phthalate (DEHP) and di-butyl phthalate (DBP) significantly induced spinal defects, such as inhibited spontaneous movement at 24 h post-fertilization (hpf), spine curvature and body length decrease at 96 hpf. The transcriptional level of the genes that are related to the development of the notochord (col8a1a and ngs), muscle (stac3, klhl41a and smyd2b) and skeleton (bmp2, spp1) were significantly altered by DEHP and DBP at 50 and 250 μg/L, which might be associated with the observed morphological changes. Notably, DBP and DEHP altered the locomotor activity of zebrafish larvae at 144 hpf, which might be due to the abnormal development of the spine and skeletal system. In conclusion, phthalates caused spinal birth defects in zebrafish embryos, induced transcriptional alterations of the spinal developmental genes, and led to abnormal behavior.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping