PUBLICATION
Methylnitrosourea, dimethylbenzanthracene and benzoapyrene differentially affect redox pathways, apoptosis and immunity in zebrafish
- Authors
- Eğimezer, G., Üstündağ, Ü.V., Ateş, P.S., Ünal, I., Üstündağ, F.D., Alturfan, A.A., Emekli-Alturfan, E., Altinoz, M.A., Elmaci, I.
- ID
- ZDB-PUB-200216-2
- Date
- 2020
- Source
- Human & Experimental Toxicology 39(7): 920-929 (Journal)
- Registered Authors
- Ateş, Perihan Seda, Emekli-Alturfan, Ebru, Üstündağ, Ünsal Veli
- Keywords
- Methylnitrosourea, antioxidant, apoptosis, benzoapyrene, dimethylbenzanthracene, immunity, zebrafish
- MeSH Terms
-
- 9,10-Dimethyl-1,2-benzanthracene/toxicity*
- Animals
- Apoptosis/drug effects
- Apoptosis Regulatory Proteins/genetics
- Benzo(a)pyrene/toxicity*
- Carcinogens/toxicity*
- Embryo, Nonmammalian
- Glutathione Transferase/metabolism
- Interferon-gamma/genetics
- Methylnitrosourea/toxicity*
- Nitric Oxide/metabolism
- Oxidation-Reduction
- Oxidative Stress/drug effects
- Superoxide Dismutase/metabolism
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 32054343 Full text @ Hum. Exp. Toxicol.
Citation
Eğimezer, G., Üstündağ, Ü.V., Ateş, P.S., Ünal, I., Üstündağ, F.D., Alturfan, A.A., Emekli-Alturfan, E., Altinoz, M.A., Elmaci, I. (2020) Methylnitrosourea, dimethylbenzanthracene and benzoapyrene differentially affect redox pathways, apoptosis and immunity in zebrafish. Human & Experimental Toxicology. 39(7):920-929.
Abstract
Cancer continues to be a major cause of mortality globally. Zebrafish present suitable models for studying the mechanisms of genotoxic carcinogens. The aim of this study was to investigate the interaction between oxidant-antioxidant status, apoptosis and immunity in zebrafish that were exposed to three different genotoxic carcinogens methylnitrosourea, dimethylbenzanthracene, benzoapyrene and methylnitrosourea + dimethylbenzanthracene starting from early embryogenesis for 30 days. Lipid peroxidation, nitric oxide levels, superoxide dismutase and glutathione-S-transferase activities and mRNA levels of apoptosis genes p53, bax, casp3a, casp2 and immunity genes fas, tnfα and ifnγ1 were evaluated. The disruption of the oxidant-antioxidant balance accompanied by altered expressions of apoptotic and immunity related genes were observed in different levels according to the carcinogen applied. Noteworthy, ifnγ expressions decreased in all carcinogen-exposed groups. Our results will provide basic data for further carcinogenesis research in zebrafish models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping