PUBLICATION

Light-Triggered Cancer Cell Specific Targeting and Liposomal Drug Delivery in a Zebrafish Xenograft Model

Authors
Kong, L., Chen, Q., Campbell, F., Snaar-Jagalska, E., Kros, A.
ID
ZDB-PUB-200214-11
Date
2020
Source
Advanced Healthcare Materials   9(6): e1901489 (Journal)
Registered Authors
Keywords
cancer nanomedicine, embryonic zebrafish, in vivo, light activation, liposomes
MeSH Terms
  • Animals
  • Cell Line, Tumor
  • Doxorubicin
  • Drug Delivery Systems
  • Heterografts
  • Humans
  • Liposomes*
  • Nanomedicine
  • Neoplasms*
  • Zebrafish
PubMed
32052583 Full text @ Adv. Healthc. Mater.
Abstract
Cell-specific drug delivery remains a major unmet challenge for cancer nanomedicines. Here, light-triggered, cell-specific delivery of liposome-encapsulated doxorubicin to xenograft human cancer cells in live zebrafish embryos is demonstrated. This method relies on light-triggered dePEGylation of liposome surfaces to reveal underlying targeting functionality. To demonstrate general applicability of this method, light-triggered, MDA-MB-231 breast cancer cell specific targeting in vivo (embryonic zebrafish) is shown using both clinically relevant, folate-liposomes, as well as an experimental liposome-cell fusion system. In the case of liposome-cell fusion, the delivery of liposomal doxorubicin direct to the cytosol of target cancer cells results in enhanced cytotoxicity, compared to doxorubicin delivery via either folate-liposomes or free doxorubicin, as well as a significant reduction in xenograft cancer cell burden within the embryonic fish.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes