PUBLICATION
Exposure to crude oil induces retinal apoptosis and impairs visual function in fish
- Authors
- Magnuson, J., Bautista, N., Lucero, J., Lund, A., Xu, E.G., Schlenk, D., Burggren, W., Roberts, A.P.
- ID
- ZDB-PUB-200211-1
- Date
- 2020
- Source
- Environmental science & technology 54(5): 2843-2850 (Journal)
- Registered Authors
- Burggren, Warren
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis
- Petroleum*
- Petroleum Pollution*
- Polycyclic Aromatic Hydrocarbons*
- Water Pollutants, Chemical*
- Zebrafish
- PubMed
- 32036658 Full text @ Env. Sci. Tech.
Citation
Magnuson, J., Bautista, N., Lucero, J., Lund, A., Xu, E.G., Schlenk, D., Burggren, W., Roberts, A.P. (2020) Exposure to crude oil induces retinal apoptosis and impairs visual function in fish. Environmental science & technology. 54(5):2843-2850.
Abstract
Polycyclic aromatic hydrocarbons (PAHs) present in crude oil are known to impair visual development in fish. However, the underlying mechanism of PAH-induced toxicity to the visual system of fishes is not understood. Embryonic zebrafish (Danio rerio) at 4 hours post-fertilization were exposed to weathered crude oil and assessed for visual function using an optokinetic response, with subsequent samples taken for immunohistochemistry and gene expression analysis. Cardiotoxicity was also assessed by measuring heart rate, stroke volume, and cardiac output, as cardiac performance has been proposed to be a contributing factor to eye-associated malformations following oil exposure. Larvae exposed to the highest concentrations of crude oil (89.8 µg/L) exhibited an increased occurrence of bradycardia, though no changes in stroke volume or cardiac output were observed. However, genes important in eye development and phototransduction were downregulated in oil-exposed larvae, with an increased occurrence of cellular apoptosis, reduced neuronal connection, and reduced optokinetic behavioral response in zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping