ZFIN ID: ZDB-PUB-200208-5
Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy
de Medeiros, G., Kromm, D., Balazs, B., Norlin, N., Günther, S., Izquierdo, E., Ronchi, P., Komoto, S., Krzic, U., Schwab, Y., Peri, F., de Renzis, S., Leptin, M., Rauzi, M., Hufnagel, L.
Date: 2020
Source: Scientific Reports   10: 1942 (Journal)
Registered Authors: Leptin, Maria, Peri, Francesca
Keywords: none
MeSH Terms: none
PubMed: 32029815 Full text @ Sci. Rep.
Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.