PUBLICATION
Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy
- Authors
- de Medeiros, G., Kromm, D., Balazs, B., Norlin, N., Günther, S., Izquierdo, E., Ronchi, P., Komoto, S., Krzic, U., Schwab, Y., Peri, F., de Renzis, S., Leptin, M., Rauzi, M., Hufnagel, L.
- ID
- ZDB-PUB-200208-5
- Date
- 2020
- Source
- Scientific Reports 10: 1942 (Journal)
- Registered Authors
- Leptin, Maria, Peri, Francesca
- Keywords
- none
- MeSH Terms
-
- Animals
- Drosophila melanogaster/physiology
- Imaging, Three-Dimensional/methods
- Infrared Rays
- Lasers
- Microscopy*
- Zebrafish/physiology
- PubMed
- 32029815 Full text @ Sci. Rep.
Citation
de Medeiros, G., Kromm, D., Balazs, B., Norlin, N., Günther, S., Izquierdo, E., Ronchi, P., Komoto, S., Krzic, U., Schwab, Y., Peri, F., de Renzis, S., Leptin, M., Rauzi, M., Hufnagel, L. (2020) Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Scientific Reports. 10:1942.
Abstract
Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping