PUBLICATION
Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution
- Authors
- Chakraborty, T., Driscoll, M.K., Jeffery, E., Murphy, M.M., Roudot, P., Chang, B.J., Vora, S., Wong, W.M., Nielson, C.D., Zhang, H., Zhemkov, V., Hiremath, C., De La Cruz, E.D., Yi, Y., Bezprozvanny, I., Zhao, H., Tomer, R., Heintzmann, R., Meeks, J.P., Marciano, D.K., Morrison, S.J., Danuser, G., Dean, K.M., Fiolka, R.
- ID
- ZDB-PUB-200207-1
- Date
- 2019
- Source
- Nature Methods 16: 1109-1113 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Mice
- Microscopy, Fluorescence/methods*
- Zebrafish
- PubMed
- 31673159 Full text @ Nat. Methods
Citation
Chakraborty, T., Driscoll, M.K., Jeffery, E., Murphy, M.M., Roudot, P., Chang, B.J., Vora, S., Wong, W.M., Nielson, C.D., Zhang, H., Zhemkov, V., Hiremath, C., De La Cruz, E.D., Yi, Y., Bezprozvanny, I., Zhao, H., Tomer, R., Heintzmann, R., Meeks, J.P., Marciano, D.K., Morrison, S.J., Danuser, G., Dean, K.M., Fiolka, R. (2019) Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. Nature Methods. 16:1109-1113.
Abstract
We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell-cell interactions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping