ZFIN ID: ZDB-PUB-200129-9
Optic cup morphogenesis requires neural crest-mediated basement membrane assembly
Bryan, C.D., Casey, M.A., Pfeiffer, R.L., Jones, B.W., Kwan, K.M.
Date: 2020
Source: Development (Cambridge, England)   147(4): (Journal)
Registered Authors: Kwan, Kristen
Keywords: Basement membrane, Extracellular matrix, Eye, Morphogenesis, Neural crest, Nidogen
MeSH Terms:
  • Alleles
  • Animals
  • Basement Membrane/embryology*
  • CRISPR-Cas Systems
  • Calcium-Binding Proteins/physiology
  • Cell Movement
  • Electrophoresis, Capillary
  • Extracellular Matrix/physiology
  • Extracellular Matrix Proteins/physiology
  • Eye/embryology*
  • Forkhead Transcription Factors/physiology
  • Gene Expression Regulation, Developmental
  • Genotype
  • Mesoderm/embryology
  • Microscopy, Electron, Transmission
  • Morphogenesis
  • Mutation
  • Neural Crest/cytology
  • Neural Crest/embryology*
  • Organogenesis
  • Retina/embryology
  • Retinal Pigment Epithelium/embryology
  • Signal Transduction
  • Transcription Factor AP-2/physiology
  • Zebrafish
  • Zebrafish Proteins/physiology
PubMed: 31988185 Full text @ Development
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ABSTRACT
Organogenesis requires precise interactions between a developing tissue and its environment. In vertebrates, the developing eye is surrounded by a complex extracellular matrix as well as multiple mesenchymal cell populations. Disruptions to either the matrix or periocular mesenchyme can cause defects in early eye development, yet in many cases, the underlying mechanism is unknown. Here, using multidimensional imaging and computational analyses in zebrafish, we establish that cell movements in the developing optic cup require neural crest. Ultrastructural analysis reveals that basement membrane formation around the developing eye is also dependent on neural crest, but only specifically around the retinal pigment epithelium. Neural crest cells produce the extracellular matrix protein nidogen: impairing nidogen function disrupts eye development, and strikingly, expression of nidogen in the absence of neural crest partially restores optic cup morphogenesis. These results demonstrate that eye formation is regulated in part by extrinsic control of extracellular matrix assembly.
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