PUBLICATION
The progestin norethindrone alters growth, reproductive histology and gene expression in zebrafish (Danio rerio)
- Authors
- Liang, Y.Q., Jing, Z., Pan, C.G., Lin, Z., Zhen, Z., Hou, L., Dong, Z.
- ID
- ZDB-PUB-200104-2
- Date
- 2020
- Source
- Chemosphere 242: 125285 (Journal)
- Registered Authors
- Keywords
- Endocrine disruption, Fish, Gene transcription, Norethindrone, Progestin
- MeSH Terms
-
- Animals
- Down-Regulation
- Female
- Gene Expression/drug effects*
- Germ Cells/drug effects
- Gonads/drug effects
- Male
- Norethindrone/toxicity*
- Ovary/drug effects
- Progestins/toxicity*
- Reproduction/drug effects
- Sex Differentiation/drug effects
- Testis/drug effects
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish Proteins/genetics*
- PubMed
- 31896209 Full text @ Chemosphere
Citation
Liang, Y.Q., Jing, Z., Pan, C.G., Lin, Z., Zhen, Z., Hou, L., Dong, Z. (2020) The progestin norethindrone alters growth, reproductive histology and gene expression in zebrafish (Danio rerio). Chemosphere. 242:125285.
Abstract
The aim of this study was to assess the long-term effects of synthetic progestin norethindrone (NET) on the growth, reproductive histology, and transcriptional expression profiles of genes associated with the hypothalamic-pituitary-gonadal (HPG) axis and germ cells in adult zebrafish. Adult zebrafish were exposed to 7, 84 and 810 ng/L NET for 90 days. The results showed that exposure to 810 ng/L NET caused a significant decrease in growth of females and males. The ovary weight and GSI was significantly reduced by NET at concentrations of 84 or 810 ng/L, which came along with the delay of ovary maturation in females. However, NET at all treatments resulted in acceleration of sperm maturation in males. In the ovaries of females, a strong inhibition of cyp19a1a gene was observed following exposure to NET at 810 ng/L. Similarly, NET at the highest treatment led to a significant down-regulation of cyp17, cyp19a1a, vasa, nanos1, dazl and dmc1 genes in the testes of males. Taken together, the overall results demonstrated that NET could impact growth and gonadal maturation, with significant alterations of transcriptional expression genes along HPG axis and germ cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping