PUBLICATION
Molecular cloning of two kcnk3 genes from the Northern snakehead (Channa argus) for quantification of their transcriptions in response to fasting and refeeding
- Authors
- Wen, Z.Y., Wang, J., Bian, C., Zhang, X., Li, J., Peng, Y., Zhan, Q., Shi, Q., Li, Y.Y.
- ID
- ZDB-PUB-191218-7
- Date
- 2019
- Source
- General and comparative endocrinology 281: 49-57 (Journal)
- Registered Authors
- Keywords
- Fasting, Northern snakehead (Channa argus), Phylogenetic analysis, Refeeding, Tissue distribution, kcnk3
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary/genetics
- Fasting/physiology*
- Feeding Behavior*
- Fishes/genetics*
- Genome
- Phylogeny
- Potassium Channels, Tandem Pore Domain/chemistry
- Potassium Channels, Tandem Pore Domain/genetics*
- Potassium Channels, Tandem Pore Domain/metabolism
- Synteny/genetics
- Tissue Distribution
- Transcription, Genetic*
- Zebrafish/genetics
- PubMed
- 31121162 Full text @ Gen. Comp. Endocrinol.
Citation
Wen, Z.Y., Wang, J., Bian, C., Zhang, X., Li, J., Peng, Y., Zhan, Q., Shi, Q., Li, Y.Y. (2019) Molecular cloning of two kcnk3 genes from the Northern snakehead (Channa argus) for quantification of their transcriptions in response to fasting and refeeding. General and comparative endocrinology. 281:49-57.
Abstract
Potassium channel subfamily K member 3 (KCNK3) has been reported to play important roles in membrane potential conduction, pulmonary hypertension and thermogenesis regulation in mammals. However, its roles remain largely unknown and scarce reports were seen in fish. In the present study, we for the first time identified two kcnk3 genes (kcnk3a and kcnk3b) from the carnivorous Northern snakehead (Channa argus) by molecular cloning and a genomic survey. Subsequently, their transcription changes in response to different feeding status were investigated. Full-length coding sequences of the kcnk3a and kcnk3b genes are 1203 and 1176 bp, encoding 400 and 391 amino acids, respectively. Multiple alignments, 3D-structure prediction and phylogenetic analysis further suggested that these kcnk3 genes may be highly conserved in vertebrates. Tissue distribution analysis by real-time PCR demonstrated that both the snakehead kcnk3s were widely transcribed in majority of the examined tissues but with different distribution patterns. In a short-term (24-h) fasting experiment, we observed that brain kcnk3a and kcnk3b genes showed totally opposite transcription patterns. In a long-term (2-week) fasting and refeeding experiment, we also observed differential change patterns for the brain kcnk3 genes. In summary, our findings suggest that the two kcnk3 genes are close while present different transcription responses to fasting and refeeding. They therefore can be potentially selected as novel target genes for improvement of production and quality of this economically important fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping