PUBLICATION
Identification and innate immunity mechanism of protective immunogens from extracellular proteins of Edwardsiella tarda
- Authors
- Li, L., Song, M., Peng, B., Peng, X.X., Li, H.
- ID
- ZDB-PUB-191213-13
- Date
- 2019
- Source
- Fish & shellfish immunology 97: 41-45 (Journal)
- Registered Authors
- Keywords
- Edwardsiella tarda, Extracellular secretory proteins, Immunogens, Vaccine
- MeSH Terms
-
- Animals
- Antibodies, Bacterial/immunology
- Antigens, Bacterial/administration & dosage
- Antigens, Bacterial/immunology*
- Bacterial Proteins/administration & dosage
- Bacterial Proteins/immunology*
- Bacterial Vaccines/administration & dosage
- Bacterial Vaccines/immunology
- Edwardsiella tarda/immunology*
- Enterobacteriaceae Infections
- Fish Diseases/immunology
- Immunity, Innate*
- Recombinant Proteins/administration & dosage
- Recombinant Proteins/immunology
- Zebrafish/immunology
- PubMed
- 31830569 Full text @ Fish Shellfish Immunol.
Citation
Li, L., Song, M., Peng, B., Peng, X.X., Li, H. (2019) Identification and innate immunity mechanism of protective immunogens from extracellular proteins of Edwardsiella tarda. Fish & shellfish immunology. 97:41-45.
Abstract
One of the most important emerging pathogens in the aquaculture industry is Edwardsiella tarda, and it causes extensive losses in farmed fish globally. The identification of protective immunogens against E. tarda is increasingly valued. We previously investigated 20 recombinant proteins of 38 E. tarda extracellular secretory proteins and identified 10 as protective immunogens in a zebrafish model. Here, we clone 10 of the remaining 18 genes, and the resulting recombinant proteins are used for evaluation of immune protection. ETAE_2147 (FliK), ETAE_0654 (PpdD), and ETAE_3259 (DamX) are identified as protective immunogens. Furthermore, their protection mechanism is explored by the detection of innate immunity genes encoding IL-1b, IL-6, IL-8, C3b, and NF-κB. The three protective immunogens stimulate zebrafish to produce higher and more lasting expression of the five immunity genes than non-protective immunogens during the first 48 h of infection. In addition, these protective immunogens are prone to be regulated by host products, which is helpful for cross-talk between host and pathogen, and thus they become vaccine candidates. These results highlight the way to understand the working mechanisms of protective immunogens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping