PUBLICATION
Effect of the immobilized microcystin-LR-degrading enzyme MlrA on nodularin degradation and its immunotoxicity study
- Authors
- Wu, X., Wu, H., Gu, X., Zhang, R., Sheng, Q., Ye, J.
- ID
- ZDB-PUB-191206-19
- Date
- 2019
- Source
- Environmental pollution (Barking, Essex : 1987) 258: 113653 (Journal)
- Registered Authors
- Keywords
- CysGO-MlrA, Degradation efficiency, Free-MlrA, Immunotoxicity, MlrA, Nodularin
- MeSH Terms
-
- Animals
- Cyanobacteria
- Ecosystem
- Enzymes, Immobilized
- Microcystins/chemistry*
- Microcystins/toxicity
- Peptides, Cyclic/chemistry*
- Peptides, Cyclic/toxicity
- PubMed
- 31801670 Full text @ Environ. Pollut.
Citation
Wu, X., Wu, H., Gu, X., Zhang, R., Sheng, Q., Ye, J. (2019) Effect of the immobilized microcystin-LR-degrading enzyme MlrA on nodularin degradation and its immunotoxicity study. Environmental pollution (Barking, Essex : 1987). 258:113653.
Abstract
In freshwater ecosystems with frequent cyanobacterial blooms, the cyanobacteria toxin pollution is becoming increasingly serious. Nodularin (NOD), which has strong biological toxicity, has emerged as a new pollutant and affects the normal growth, development and reproduction of aquatic organisms. However, little information is available regarding this toxin. In this study, a graphene oxide material modified by L-cysteine was synthesized and used to immobilize microcystin-LR (MC-LR)-degrading enzyme (MlrA) to form an immobilized enzyme nanocomposite, CysGO-MlrA. Free-MlrA was used as a control. The efficiency of NOD removal by CysGO-MlrA was investigated. Additionally, the effects of CysGO-MlrA and the NOD degradation product on zebrafish lymphocytes were detected to determine the biological toxicity of these two substances. The results showed the following: (1) There was no significant difference in the degradation efficiency of NOD between CysGO-MlrA and free-MlrA; the degradation rate of both was greater than 80% at 1 h (2) The degradation efficiency of the enzyme could retain greater than 81% of the initial degradation efficiency after the CysGO-MlrA had been reused 7 times. (3) CysGO-MlrA retained greater than 50% of its activity on the 8th day when preserved at 0 °C, while free-MlrA lost 50% of its activity on the 4th day. (4) CysGO-MlrA and the degradation product of NOD showed no obvious cytotoxicity to zebrafish lymphocytes. Therefore, CysGO-MlrA might be used as an efficient and ecologically safe degradation material for NOD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping