ZFIN ID: ZDB-PUB-191206-10
Neural alterations and hyperactivity of the hypothalamic-pituitary-thyroid axis in Oatp1c1-deficiency
Admati, I., Wasserman-Bartov, T., Tovin, A., Rozenblat, R., Blitz, E., Zada, D., Lerer-Goldshtein, T., Appelbaum, L.
Date: 2019
Source: Thyroid : official journal of the American Thyroid Association   30(1): 161-174 (Journal)
Registered Authors: Admati, Inbal, Appelbaum, Lior, Blitz, Einat, Lerer-Goldshtein, Tali, Rozenblat, Rotem, Tovin, Adi, Zada, David
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Astrocytes/metabolism
  • Behavior, Animal
  • Brain/metabolism
  • Cell Membrane/metabolism
  • Disease Models, Animal
  • Endothelial Cells/metabolism
  • Gene Knockout Techniques
  • Hypothalamo-Hypophyseal System/physiology*
  • Microscopy, Fluorescence
  • Mutation*
  • Neurons/physiology*
  • Organic Anion Transporters/deficiency
  • Organic Anion Transporters/genetics*
  • Organic Anion Transporters/physiology
  • Thyroid Gland/physiology*
  • Zebrafish
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology
PubMed: 31797746 Full text @ Thyroid
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ABSTRACT
The thyroid hormones (THs) T3 and T4 are crucial regulators of brain development and function. Cell-specific transporter proteins facilitate TH uptake and efflux across the cell membrane, and insufficient TH transport causes hypothyroidism and mental retardation. Mutations in the TH transporters monocarboxylate transporter 8 (MCT8, SLC16A2) and the organic anion-transporting polypeptide 1C1 (OATP1C1, SLCO1C1) are associated with the psychomotor retardation Allan-Herndon-Dudley syndrome (AHDS) and juvenile neurodegeneration, respectively. In order to understand the mechanisms and test potential treatments for the recently discovered OATP1C1-deficiency, we established an oatp1c1 mutant (oatp1c1-/-) zebrafish. oatp1c1 is expressed in endothelial cells, neurons, and astrocytes in zebrafish. The activity of the hypothalamic-pituitary-thyroid (HPT) axis and behavioral locomotor activity increased in oatp1c1-/- larvae. Neuropathological analysis revealed structural alteration in radial glial cells and shorter neuronal axons in oatp1c1-/- larvae and adults. Notably, oatp1c1-/- and oatp1c1-/-Xmct8-/- adults exhibit an enlarged thyroid gland (goiter). Pharmacological assays showed that TH analogs, but not THs, can reduce the size and improve the color of the thyroid gland in adult mutant zebrafish. These results establish a vertebrate model for OATP1C1-deficiency that demonstrates endocrinological, neurological, and behavioral alterations mimicking findings observed in a OATP1C1-deficient patient. Furthermore, the curative effect of TH analogs in the oatp1c1-/- zebrafish model may provide a lead towards a treatment modality in human patients.
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