PUBLICATION

A morphogenetic EphB/EphrinB code controls hepatopancreatic duct formation

Authors
Thestrup, M.I., Caviglia, S., Cayuso, J., Heyne, R.L.S., Ahmad, R., Hofmeister, W., Satriano, L., Wilkinson, D.G., Andersen, J.B., Ober, E.A.
ID
ZDB-PUB-191121-3
Date
2019
Source
Nature communications   10: 5220 (Journal)
Registered Authors
Caviglia, Sara, Ober, Elke, Wilkinson, David
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Bile Ducts/embryology
  • Bile Ducts/metabolism*
  • Cell Differentiation/genetics
  • Ephrin-B1/genetics
  • Ephrin-B1/metabolism*
  • Ephrin-B3/genetics
  • Ephrin-B3/metabolism
  • Gene Expression Profiling
  • Hepatopancreas/embryology
  • Hepatopancreas/metabolism*
  • Ligands
  • Morphogenesis/genetics
  • Mutation
  • Protein Binding
  • Receptors, Eph Family/genetics
  • Receptors, Eph Family/metabolism*
  • Signal Transduction/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
31745086 Full text @ Nat. Commun.
Abstract
The hepatopancreatic ductal (HPD) system connects the intrahepatic and intrapancreatic ducts to the intestine and ensures the afferent transport of the bile and pancreatic enzymes. Yet the molecular and cellular mechanisms controlling their differentiation and morphogenesis into a functional ductal system are poorly understood. Here, we characterize HPD system morphogenesis by high-resolution microscopy in zebrafish. The HPD system differentiates from a rod of unpolarized cells into mature ducts by de novo lumen formation in a dynamic multi-step process. The remodeling step from multiple nascent lumina into a single lumen requires active cell intercalation and myosin contractility. We identify key functions for EphB/EphrinB signaling in this dynamic remodeling step. Two EphrinB ligands, EphrinB1 and EphrinB2a, and two EphB receptors, EphB3b and EphB4a, control HPD morphogenesis by remodeling individual ductal compartments, and thereby coordinate the morphogenesis of this multi-compartment ductal system.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping