PUBLICATION
Waterborne microcystin-LR exposure induced chronic inflammatory response via MyD88-dependent toll-like receptor signaling pathway in male zebrafish
- Authors
- Lin, W., Guo, H., Wang, L., Zhang, D., Wu, X., Li, L., Qiu, Y., Yang, L., Li, D., Tang, R.
- ID
- ZDB-PUB-191112-10
- Date
- 2019
- Source
- The Science of the total environment 702: 134969 (Journal)
- Registered Authors
- Keywords
- Gene expression, Inflammation, MC-LR, TLR/MyD88 signaling pathway, Zebrafish
- MeSH Terms
-
- Animals
- Male
- Microcystins/toxicity*
- Myeloid Differentiation Factor 88/metabolism*
- Water Pollutants, Chemical/toxicity*
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 31710851 Full text @ Sci. Total Environ.
Citation
Lin, W., Guo, H., Wang, L., Zhang, D., Wu, X., Li, L., Qiu, Y., Yang, L., Li, D., Tang, R. (2019) Waterborne microcystin-LR exposure induced chronic inflammatory response via MyD88-dependent toll-like receptor signaling pathway in male zebrafish. The Science of the total environment. 702:134969.
Abstract
Waterborne microcystin-LR (MC-LR) released by cyanobacterial blooms in eutrophic water bodies have caused serious risk to aquatic animal and human health. In the present study, we for the first time conducted a comprehensive in vivo investigation on chronic inflammatory responses and its molecular pathways of different environmental relevant levels of MC-LR (0, 0.4, 2 and 10 μg/L) in male zebrafish (Danio rerio). The results showed that chronic MC-LR exposure caused splenic inflammatory changes including the formation of melano-macrophage centers, remarkable elevation of serum tumor necrosis factor alpha (TNFα) and interleukin 1 beta (IL1β) levels as well as significant upregulated expression of MyD88-dependent toll-like receptor (TLR/MyD88) signaling pathway genes (tlr4a, myd88, erk2, p38a, il1β and tnfα). The immunohistochemical and western blot results further validated that higher MC-LR concentrations tended to enhance the MyD88 signal. Moreover, significant decreases of serum C3 levels along with splenic c3b expression in the 10 μg/L exposure group proved that chronic MC-LR exposure could ultimately decrease the innate immunity of fish. Our findings revealed that chronic exposure of MC-LR could cause chronic inflammation through TLR/MyD88 signaling pathway and subsequently induce immune disorders in male zebrafish, which also urge us to pay more attention on the potential immunotoxicity of long-term exposure to low concentration of MC-LR.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping