PUBLICATION
Induction of time- and dose-dependent oxidative stress of triazophos to brain and liver in zebrafish (Danio rerio)
- Authors
- Wang, G., Xiong, D., Wu, M., Wang, L., Yang, J.
- ID
- ZDB-PUB-191029-12
- Date
- 2019
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 228: 108640 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Enzyme activity, Gene expression, Lipid peroxidation, Oxidative stress, Triazophos
- MeSH Terms
-
- Animals
- Brain/metabolism*
- Catalase/metabolism
- Glutathione/metabolism
- Lipid Peroxidation
- Liver/metabolism*
- Malondialdehyde/metabolism
- Organothiophosphates/toxicity*
- Oxidative Stress
- Reactive Oxygen Species/metabolism*
- Superoxide Dismutase/metabolism
- Triazoles/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism*
- PubMed
- 31654829 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Wang, G., Xiong, D., Wu, M., Wang, L., Yang, J. (2019) Induction of time- and dose-dependent oxidative stress of triazophos to brain and liver in zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 228:108640.
Abstract
Extensive use of triazophos for the chemical control of pests in agriculture or aquaculture might strongly disturb the aquatic environment due to residue accumulation through various routes like surface run-off, spray-drift and effluent from factories, which have potential negative effects to non-target aquatic organisms. Previous studies have documented the antioxidative effects of triazophos to mammals, however, the oxidative toxicity of triazophos to fish has not been adequately studied to date. Thus, an acute exposure (96 h) to triazophos at different concentrations of 0.06, 0.3 and 1.5 mg/L (corresponding to 1/50th, 1/10th and 1/2th of 96 h-LC50, respectively), was conducted to investigate the triazophos-induced oxidative stress in adult zebrafish (Danio rerio). The results showed that the time- and dose-dependent induction of oxidative stress except for the ROS level in liver after 24 and 48 h exposure, as indicated by increased reactive oxygen species (ROS), malondialdehyde (MDA) level and a compromised antioxidant defense system, including increased superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione (GSH) content as well as the increased at first and decreased afterwards genes expression (Sod1, Sod2, Cat and Gpx) in brain. Simultaneously, ROS and MDA showed an increased trend, SOD activity, CAT activity and GSH content showed a trend of increasing at 24 h and decreasing at 48 h, and then increasing at 96 h and Sod1, Sod2, Cat and Gpx gene showed decreasing at first and then increasing in liver tissue. The present study concluded that the damage of the antioxidant system by triazophos induced oxidative stress in the brain and liver of zebrafish with concomitant lipid peroxidation, which is an important mechanism underlying the triazophos-induced acute toxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping