PUBLICATION
Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio)
- Authors
- Harjula, S.E., Saralahti, A.K., Ojanen, M.J.T., Rantapero, T., Uusi-Mäkelä, M.I.E., Nykter, M., Lohi, O., Parikka, M., Rämet, M.
- ID
- ZDB-PUB-191022-19
- Date
- 2019
- Source
- Developmental and comparative immunology 103: 103523 (Journal)
- Registered Authors
- Harjula, Sanna, Rämet, Mika, Saralahti, Anni, Uusi-Mäkelä, Meri
- Keywords
- Forward genetic screen, Mycobacterium marinum, Transcriptome analysis, Zebrafish
- Datasets
- GEO:GSE118350, GEO:GSE118288
- MeSH Terms
-
- Animals
- Gene Expression Profiling
- Hematopoietic System/immunology*
- Kidney/immunology
- Mycobacterium Infections, Nontuberculous/immunology*
- Mycobacterium marinum
- Zebrafish/immunology*
- PubMed
- 31626817 Full text @ Dev. Comp. Immunol.
Citation
Harjula, S.E., Saralahti, A.K., Ojanen, M.J.T., Rantapero, T., Uusi-Mäkelä, M.I.E., Nykter, M., Lohi, O., Parikka, M., Rämet, M. (2019) Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio). Developmental and comparative immunology. 103:103523.
Abstract
Tuberculosis remains a major global health challenge. To gain information about genes important for defense against tuberculosis, we used a well-established tuberculosis model; Mycobacterium marinum infection in adult zebrafish. To characterize the immunological response to mycobacterial infection at 14 days post infection, we performed a whole-genome level transcriptome analysis using cells from kidney, the main hematopoietic organ of adult zebrafish. Among the upregulated genes, those associated with immune signaling and regulation formed the largest category, whereas the largest group of downregulated genes had a metabolic role. We also performed a forward genetic screen in adult zebrafish and identified a fish line with severely impaired survival during chronic mycobacterial infection. Based on transcriptome analysis, these fish have decreased expression of several immunological genes. Taken together, these results give new information about the genes involved in the defense against mycobacterial infection in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping