PUBLICATION
Lethal toxicity and gene expression changes in embryonic zebrafish upon exposure to individual and mixture of malathion, chlorpyrifos and lambda-cyhalothrin
- Authors
- Shen, W., Lou, B., Xu, C., Yang, G., Yu, R., Wang, X., Li, X., Wang, Q., Wang, Y.
- ID
- ZDB-PUB-190916-1
- Date
- 2019
- Source
- Chemosphere 239: 124802 (Journal)
- Registered Authors
- Keywords
- Aquatic toxicity, Gene expressions, Mixture toxicity, Synergistic response
- MeSH Terms
-
- Animals
- Chlorpyrifos/toxicity
- Complex Mixtures/toxicity*
- Drug Interactions
- Endocrine Disruptors/toxicity
- Larva/drug effects*
- Life Cycle Stages/drug effects
- Malathion/toxicity
- Nitriles/toxicity
- Pesticides/toxicity*
- Pyrethrins/toxicity
- Zebrafish/embryology
- Zebrafish/metabolism
- PubMed
- 31521933 Full text @ Chemosphere
- CTD
- 31521933
Citation
Shen, W., Lou, B., Xu, C., Yang, G., Yu, R., Wang, X., Li, X., Wang, Q., Wang, Y. (2019) Lethal toxicity and gene expression changes in embryonic zebrafish upon exposure to individual and mixture of malathion, chlorpyrifos and lambda-cyhalothrin. Chemosphere. 239:124802.
Abstract
Pesticides are usually present as mixtures in water environments. Evaluating the toxic effects of individual pesticide may not be enough for protecting ecological environment due to interactions among substances. In this study, we aimed to examine the lethal doses and gene expression changes in zebrafish (Danio rerio) upon exposure to individual and mixture pesticides [malathion (MAL), chlorpyrifos (CHL) and lambda-cyhalothrin (LCY)]. Individual pesticide toxicity evaluation manifested that the toxicity of the three pesticides to D. rerio at various developmental stages (embryonic, larval, juvenile and adult stages) followed the order of LCY > CHL > MAL. On the contrary, the least toxicity to the animals was discovered from MAL. Most of the tested pesticides displayed lower toxicities to the embryonic stage compared with other life stages of zebrafish. Synergistic effects were monitored from two binary mixtures of LCY in combination with MAL or CHL and ternary mixture of MAL + CHL + LCY. The expressions of 16 genes involved in oxidative stress, immunity system, cell apoptosis and endocrine disruption at the mRNA level revealed that embryonic zebrafish were influenced by the individual or mixture pesticides. The expressions of Tnf, P53, TRα, Crh and Cyp19a exerted greater variations upon exposure to pesticide mixtures compared with their individual compounds. Collectively, the transcriptional responses of these genes might afford early warning biomarkers for identifying pollutant exposure, and the data acquired from this study provided valuable insights into the comprehensive toxicity of pesticide mixtures to zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping