PUBLICATION
Inhibited Lipophagy Suppresses Lipid Metabolism in Zebrafish Liver Cells
- Authors
- Wang, J., Han, S.L., Lu, D.L., Li, L.Y., Limbu, S.M., Li, D.L., Zhang, M.L., Du, Z.Y.
- ID
- ZDB-PUB-190910-5
- Date
- 2019
- Source
- Frontiers in Physiology 10: 1077 (Journal)
- Registered Authors
- Du, Zhen-Yu, Li, Dong-Liang, Limbu, Samwel Michele, Lu, Dong-Liang, Zhang, Mei-Ling
- Keywords
- esterification, fatty acid β-oxidation, lipid metabolism, lipophagy, zebrafish liver cells
- MeSH Terms
- none
- PubMed
- 31496957 Full text @ Front. Physiol.
Citation
Wang, J., Han, S.L., Lu, D.L., Li, L.Y., Limbu, S.M., Li, D.L., Zhang, M.L., Du, Z.Y. (2019) Inhibited Lipophagy Suppresses Lipid Metabolism in Zebrafish Liver Cells. Frontiers in Physiology. 10:1077.
Abstract
Lipophagy degrades lipid droplets (LDs) through the lysosomal degradative pathway, thus plays important roles in regulating lipid metabolism in mammals. However, information on the existence and functions of lipophagy in fish lipid metabolism is still limited. In the present study, we confirmed the existence of lipophagy by observing the structures of LDs sequestered in autophagic vacuoles in the zebrafish liver cell line (ZFL) via electronic microscopy. Moreover, starved cells increased the mRNA expression of the microtubule-associated protein 1A/1B light chain 3 beta (LC3), which is a marker protein for autophagy and protein conversion from LC3-I to LC3-II. Inhibiting autophagy with chloroquine increased significantly the LDs content and decreased fatty acid β-oxidation and esterification activities in the ZFL cells cultured in the fed state. Furthermore, inhibiting autophagy function downregulated the mRNA expression of the genes and their proteins related to lipid metabolism. Altogether, the present study verified the existence of lipophagy and its essential regulatory roles in lipid metabolism in fish cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping