PUBLICATION
Isolation, identification and characterisation of an emerging fish pathogen, Acinetobacter pittii, from diseased loach (Misgurnus anguillicaudatus) in China
- Authors
- Wang, X., Li, J., Cao, X., Wang, W., Luo, Y.
- ID
- ZDB-PUB-190822-4
- Date
- 2019
- Source
- Antonie van Leeuwenhoek 113(1): 21-32 (Journal)
- Registered Authors
- Keywords
- Acinetobacter pittii, Fish pathogen, Misgurnus anguillicaudatus, Multilocus sequence typing, Pathogenicity
- MeSH Terms
-
- Acinetobacter/classification*
- Acinetobacter/genetics*
- Acinetobacter/isolation & purification
- Animals
- Bacterial Proteins/genetics
- China
- Cypriniformes/microbiology*
- Fish Diseases/microbiology
- Multilocus Sequence Typing
- Phylogeny
- RNA, Ribosomal, 16S/genetics
- PubMed
- 31432291 Full text @ Antonie Van Leeuwenhoek
Citation
Wang, X., Li, J., Cao, X., Wang, W., Luo, Y. (2019) Isolation, identification and characterisation of an emerging fish pathogen, Acinetobacter pittii, from diseased loach (Misgurnus anguillicaudatus) in China. Antonie van Leeuwenhoek. 113(1):21-32.
Abstract
Although members of the genus Acinetobacter have emerged as important nosocomial pathogens causing severe human infections, there are few reports about their occurrence as fish pathogens. In this study, five bacterial strains were isolated from diseased loach (Misgurnus anguillicaudatus) cultured in a farm in China. The diseased loach displayed shedding of skin mucus and many petechial haemorrhages all over the body. Based on sequence analyses of 16S rRNA and rpoB genes, the isolates were identified as Acinetobacter pittii. An experimental infection assay confirmed their pathogenicity to loach. The results of artificial infection in zebrafish (Barchydanio rerio) and nematode (Caenorhabditis elegans) suggested that, as well as loach, these A. pittii isolates are pathogenic and highly virulent to these organisms. Multilocus sequence typing analysis revealed that all the isolates belong to sequence type (ST) 839, which may be the dominant clone causing fish disease and exhibits a close phylogenetic relationship with ST396 from human clinical samples in Korea or Taiwan China. This is the first report demonstrating that A. pittii is an emerging causal agent of mass mortality in loach and poses significant risks to fish culturing besides causing human clinical infection worldwide.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping