PUBLICATION
The mechanisms underlying the developmental effects of bisphenol F on zebrafish
- Authors
- Mu, X., Liu, J., Yuan, L., Yang, K., Huang, Y., Wang, C., Yang, W., Shen, G., Li, Y.
- ID
- ZDB-PUB-190816-1
- Date
- 2019
- Source
- The Science of the total environment 687: 877-884 (Journal)
- Registered Authors
- Keywords
- Bisphenol F, Depigmentation, Developmental effects, Neurotoxicity, Zebrafish embryos
- MeSH Terms
-
- Animals
- Benzhydryl Compounds/toxicity*
- Embryonic Development/drug effects*
- Larva
- Phenols/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 31412491 Full text @ Sci. Total Environ.
Citation
Mu, X., Liu, J., Yuan, L., Yang, K., Huang, Y., Wang, C., Yang, W., Shen, G., Li, Y. (2019) The mechanisms underlying the developmental effects of bisphenol F on zebrafish. The Science of the total environment. 687:877-884.
Abstract
With the increasing use of bisphenol F (BPF) as BPA alternative, BPF are widely distributed in multiple environment media. Our previous study demonstrated that BPF possess equivalent toxicity towards zebrafish as BPA, while its toxic mechanism remains largely unknown. To investigate the mechanisms mediating the developmental effects of BPF, zebrafish embryos were exposed to 0.0005, 0.5, and 5.0 mg/L BPF. Morphological examination indicated that BPF exposure led to depigmentation, decreased heart rate, inhibited spontaneous movement, hatch inhibition, and spinal deformation. Motor neuron-green fluorescence zebrafish assay indicated that exposure to 0.5 or 5.0 mg/L BPF affected embryonic motor neuron development, which is consistent with the spinal defect and spontaneous movement inhibition. Transcriptomic analysis showed that genes associated with the observed symptoms, including neuron development (ngln2a, socs3a, fosb), cardiac development (klf2a), and spinal deformation (ngs, col8a1a, egr2a), were down-regulated after exposure to either 0.0005 (environmental relevant concentration) or 0.5 mg/L BPF. This partially explained the mechanisms underlying the effects of BPF. In conclusion, BPF had the potential to affect zebrafish development even at environmental level through down-regulating associated genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping