PUBLICATION

Unlimited Genetic Switches for Cell-Type-Specific Manipulation

Authors
Garcia-Marques, J., Yang, C.P., Espinosa-Medina, I., Mok, K., Koyama, M., Lee, T.
ID
ZDB-PUB-190810-2
Date
2019
Source
Neuron   104(2): 227-238.e7 (Journal)
Registered Authors
Keywords
CRISPR/Cas9, CaSSA, Drosophila, cell-type-specific, gene trap, genetic access, genetic intersection, recombinase, single-strand annealing, zebrafish
MeSH Terms
  • Animals
  • CRISPR-Cas Systems*
  • DNA Repair*
  • Drosophila
  • Gene Targeting/methods*
  • Genetic Techniques
  • RNA, Guide, Kinetoplastida
  • Recombinases/genetics
  • Zebrafish
PubMed
31395429 Full text @ Neuron
Citation
Garcia-Marques, J., Yang, C.P., Espinosa-Medina, I., Mok, K., Koyama, M., Lee, T. (2019) Unlimited Genetic Switches for Cell-Type-Specific Manipulation. Neuron. 104(2):227-238.e7.
Abstract
Gaining independent genetic access to discrete cell types is critical to interrogate their biological functions as well as to deliver precise gene therapy. Transcriptomics has allowed us to profile cell populations with extraordinary precision, revealing that cell types are typically defined by a unique combination of genetic markers. Given the lack of adequate tools to target cell types based on multiple markers, most cell types remain inaccessible to genetic manipulation. Here we present CaSSA, a platform to create unlimited genetic switches based on CRISPR/Cas9 (Ca) and the DNA repair mechanism known as single-strand annealing (SSA). CaSSA allows engineering of independent genetic switches, each responding to a specific gRNA. Expressing multiple gRNAs in specific patterns enables multiplex cell-type-specific manipulations and combinatorial genetic targeting. CaSSA is a new genetic tool that conceptually works as an unlimited number of recombinases and will facilitate genetic access to cell types in diverse organisms.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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