PUBLICATION

Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models

Authors
Rhee, J., Han, E., Nam, K.J., Lim, K.H., Chan Rah, Y., Park, S., Koun, S., Park, H.C., Choi, J.
ID
ZDB-PUB-190803-15
Date
2019
Source
International journal of pediatric otorhinolaryngology   126: 109611 (Journal)
Registered Authors
Koun, Soonil, Park, Hae-Chul
Keywords
Hair cell, Particulate matter, Toxicity, Zebrafish
MeSH Terms
  • Animals
  • Cell Count
  • Embryo, Nonmammalian
  • Hair Cells, Auditory/pathology*
  • Heart Rate
  • Models, Animal
  • Particulate Matter/toxicity*
  • Zebrafish/embryology*
PubMed
31374386 Full text @ Int. J. Pediatr. Otorhinolaryngol.
Abstract
Particulate matter (PM) exposure has become one of the most serious problems. The aim of the present study was to evaluate the hair cell damage and possible developmental toxicity caused by PM2.5 exposure using a zebrafish model.
Zebrafish embryos were exposed to various concentrations of PM2.5. Developmental toxicity was evaluated based on general morphology score (GMS) system and Panzica-Kelly score, and by measurement of body length and heart rate. To evaluate hair cell damage, the average number of total hair cells within four neuromasts exposed to various concentrations of PM2.5 was compared with that of the control group.
Morphological abnormalities evaluated by the GMS system and Panzica-Kelly score were rare and body length tended to be shorter in the PM2.5-exposed groups. Heart rate decreased significantly in the PM2.5-exposed group. Additionally, significant hair cell damage was observed after PM2.5 exposure. It was dose-dependent and more severe after a longer period exposure (10 dpf).
In zebrafish embryos, exposure of PM2.5 in the early stages of life decreased heart rate and caused significant hair cell damage in a dose-dependent manner.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes