ZFIN ID: ZDB-PUB-190803-10
Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish
Zhang, Z., Ran, C., Ding, Q.W., Liu, H.L., Xie, M.X., Yang, Y.L., Xie, Y.D., Gao, C.C., Zhang, H.L., Zhou, Z.G.
Date: 2019
Source: Communications biology   2: 274 (Journal)
Registered Authors: Ding, Qianwen, Gao, Chenchen, Ran, Chao, Xie, Yadong, Yang, Yalin, Zhang, Zhen, Zhou, Zhigang
Keywords: Antimicrobials, Dysbiosis, Microbiota, Non-alcoholic fatty liver disease
MeSH Terms:
  • Animals
  • Antimicrobial Cationic Peptides/biosynthesis
  • Antimicrobial Cationic Peptides/metabolism*
  • Diet, High-Fat
  • Fatty Liver/etiology*
  • Gastrointestinal Microbiome
  • Hypoxia-Inducible Factor 1, alpha Subunit/metabolism*
  • Lactobacillus
  • Larva
  • Polysaccharides/administration & dosage*
  • Prebiotics/administration & dosage*
  • Zebrafish/growth & development
PubMed: 31372513 Full text @ Commun Biol
Natural polysaccharides have received much attention for their ability to ameliorate hepatic steatosis induced by high-fat diet. However, the potential risks of their use have been less investigated. Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury. This is due to the fact that BL23 EPS induces gut microbial dysbiosis, while LGG EPS promotes microbial homeostasis. We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota. Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.