PUBLICATION

Retention of paternal DNA methylome in the developing zebrafish germline

Authors
Skvortsova, K., Tarbashevich, K., Stehling, M., Lister, R., Irimia, M., Raz, E., Bogdanovic, O.
ID
ZDB-PUB-190713-2
Date
2019
Source
Nature communications   10: 3054 (Journal)
Registered Authors
Bogdanovic, Ozren, Irimia, Manuel, Raz, Erez, Tarbashevich, Katsiyarina
Keywords
none
Datasets
GEO:GSE122723, GEO:GSE122480, GEO:GSE123493, GEO:GSE122722, GEO:GSE128986
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • DNA Methylation*
  • Embryo, Nonmammalian
  • Embryonic Development/genetics
  • Epigenesis, Genetic/physiology
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental/genetics*
  • Germ Cells/growth & development*
  • Paternal Inheritance*
  • Promoter Regions, Genetic/genetics
  • Whole Genome Sequencing
  • Zebrafish/genetics*
PubMed
31296860 Full text @ Nat. Commun.
Abstract
Two waves of DNA methylation reprogramming occur during mammalian embryogenesis; during preimplantation development and during primordial germ cell (PGC) formation. However, it is currently unclear how evolutionarily conserved these processes are. Here we characterise the DNA methylomes of zebrafish PGCs at four developmental stages and identify retention of paternal epigenetic memory, in stark contrast to the findings in mammals. Gene expression profiling of zebrafish PGCs at the same developmental stages revealed that the embryonic germline is defined by a small number of markers that display strong developmental stage-specificity and that are independent of DNA methylation-mediated regulation. We identified promoters that are specifically targeted by DNA methylation in somatic and germline tissues during vertebrate embryogenesis and that are frequently misregulated in human cancers. Together, these detailed methylome and transcriptome maps of the zebrafish germline provide insight into vertebrate DNA methylation reprogramming and enhance our understanding of the relationships between germline fate acquisition and oncogenesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping