ZFIN ID: ZDB-PUB-190705-4
Skeletal mineralisation in association with type X collagen expression is an ancestral feature for jawed vertebrates
Debiais-Thibaud, M., Simion, P., Ventéo, S., Muñoz, D., Marcellini, S., Mazan, S., Haitina, T.
Date: 2019
Source: Molecular Biology and Evolution   36(10): 2265-2276 (Journal)
Registered Authors: Debiais-Thibaud, Mélanie
Keywords: chondrichthyan, mineralisation, scales, teeth, type X collagen
MeSH Terms:
  • Animals
  • Calcification, Physiologic/genetics*
  • Collagen Type X/genetics*
  • Collagen Type X/metabolism
  • Elasmobranchii/genetics*
  • Elasmobranchii/metabolism
  • Gene Duplication
  • Phylogeny
  • Synteny
PubMed: 31270539 Full text @ Mol Bio Evol
In order to characterize the molecular bases of mineralising cell evolution, we targeted type X collagen, a non-fibrillar network forming collagen encoded by the Col10a1 gene. It is involved in the process of endochondral ossification in ray-finned fishes and tetrapods (Osteichthyes), but until now unknown in cartilaginous fishes (Chondrichthyes). We show that holocephalans and elasmobranchs have respectively five and six tandemly duplicated Col10a1 gene copies that display conserved genomic synteny with osteichthyan Col10a1 genes. All Col10a1 genes in the catshark Scyliorhinus canicula are expressed in ameloblasts and/or odontoblasts of teeth and scales, during the stages of extracellular matrix protein secretion and mineralisation. Only one duplicate is expressed in the endoskeletal (vertebral) mineralising tissues. We also show that the expression of type X collagen is present in teeth of two osteichthyans, the zebrafish Danio rerio and the western clawed frog Xenopus tropicalis, indicating an ancestral jawed vertebrate involvement of type X collagen in odontode formation. Our findings push the origin of Col10a1 gene prior to the divergence of osteichthyans and chondrichthyans, and demonstrate its ancestral association with mineralisation of both the odontode skeleton and the endoskeleton.