PUBLICATION
Exposure to cadmium causes inhibition of otolith development and behavioral impairment in zebrafish larvae
- Authors
- Han, J., Liu, K., Wang, R., Zhang, Y., Zhou, B.
- ID
- ZDB-PUB-190702-8
- Date
- 2019
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 214: 105236 (Journal)
- Registered Authors
- Zhou, BingSheng
- Keywords
- Cadmium, Calcium, Locomotor and balance control, Otolith development, Zebrafish embryos/larvae
- MeSH Terms
-
- Animals
- Behavior, Animal*/drug effects
- Cadmium/toxicity*
- Calcium/analysis
- Environmental Exposure*
- Larva/drug effects
- Motor Activity/drug effects
- Otolithic Membrane/drug effects
- Otolithic Membrane/growth & development*
- Time Factors
- Toxicity Tests
- Transcription, Genetic/drug effects
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- PubMed
- 31260825 Full text @ Aquat. Toxicol.
Citation
Han, J., Liu, K., Wang, R., Zhang, Y., Zhou, B. (2019) Exposure to cadmium causes inhibition of otolith development and behavioral impairment in zebrafish larvae. Aquatic toxicology (Amsterdam, Netherlands). 214:105236.
Abstract
Otolith consisting largely of calcium carbonate, fibrous and proteins, is vital for maintaining body balance and/or hearing of fish. The formation of otolith involves Ca2+ transport and deposition. In the present study, we investigated the effects of Cd2+ on otoliths development by using zebrafish embryos as model. The results showed that exposure to Cd2+ inhibited the utricular and saccular otoliths growth, indicated by reduced lateral areas. Swimming speeds were reduced and a losing balance control was observed in Cd2+ exposed larvae. The genes related to Ca2+ transport (e.g. plasma membrane Ca2+-ATPase isoform 2, pmca2; Ca2+-ATPase isoform 2, atp2b1a) and regulation (e.g. parathyroid hormone ligand type-1, pth1; stanniocalcin isoform 1, stc1) were significantly downregulated. However, the adverse effects of Cd2+ on otoliths growth and swimming activity can be protected by supplementation of Ca2+ in exposure medium. Body burden of Cd2+ in larvae was reduced upon the supplement with Ca2+. The overall results suggest that exposure to Cd2+ can inhibit influx of Ca2+, leading to less deposition of CaCO3 for otolith growth, and finally result in impaired balance control and swimming activity in zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping