PUBLICATION
Impact of gadolinium-based contrast agents on the growth of fish cells lines
- Authors
- Parant, M., Sohm, B., Flayac, J., Perrat, E., Chuburu, F., Cadiou, C., Rosin, C., Cossu-Leguille, C.
- ID
- ZDB-PUB-190702-10
- Date
- 2019
- Source
- Ecotoxicology and environmental safety 182: 109385 (Journal)
- Registered Authors
- Keywords
- Cell growth, Contrast agent, Doratem(®), Gadolinium, Gadoteric acid, ZF4 cell line
- MeSH Terms
-
- Animals
- Biomarkers/analysis
- Cell Cycle/drug effects
- Cell Line
- Cell Survival/drug effects
- Chelating Agents
- Contrast Media/toxicity*
- Gadolinium DTPA/toxicity*
- Heterocyclic Compounds/toxicity*
- Magnetic Resonance Imaging
- Meglumine/toxicity*
- Oncorhynchus mykiss
- Organometallic Compounds/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish
- PubMed
- 31260918 Full text @ Ecotoxicol. Environ. Saf.
Citation
Parant, M., Sohm, B., Flayac, J., Perrat, E., Chuburu, F., Cadiou, C., Rosin, C., Cossu-Leguille, C. (2019) Impact of gadolinium-based contrast agents on the growth of fish cells lines. Ecotoxicology and environmental safety. 182:109385.
Abstract
The present study was the first approach conducted under environmental concentrations of Gd-DOTA and Gd-DTPA-BMA to assess cellular impacts of these compounds. Gd-DOTA (Gadoteric acid) is one of the most stable contrast agent, currently used as Dotarem® formulation during Magnetic Resonance Imaging exams. The study was mainly performed on a Zebra Fish cell line (ZF4; ATCC CRL-2050). At the concentrations of 0.127 nM and 63.59 nM (respectively 20 ng and 10 μg of Gd/L), we did not observed any toxicity of Dotarem® but a slowdown of the cell growth was clearly measured. The effect is independent of medium renewing during 6 days of cell culturing. The same effect was observed i-with Gd-DOTA on another fish cell line (RT W1 gills; ATCC CRL-2523) and ii-with another contrast agent (Gd-DTPA-BMA - Omniscan®) on ZF4 cells. On the ZF4 cell line, the diminution of the cell growth was of the same order during 20 days of exposure to a culture medium spiked with 63.59 nM of Dotarem® and was reversible within the following 8 days when Dotarem® was removed from the medium. As shown by using modified DOTA structure (Zn-DOTA), the effect may be due to the chelating structure of the contrast agent rather than to the Gd ion. Until now, the main attention concerning the impact of Gd-CA on living cells concerned the hazard due to Gd release. According to our results, quantifying the presence of Gd-CA chelating structures in aquatic environments must be also monitored.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping