PUBLICATION
Accumulation, Biotransformation, and Endocrine Disruption Effects of Fluorotelomer Surfactant Mixtures on Zebrafish
- Authors
- Shi, G., Cui, Q., Zhang, H., Cui, R., Guo, Y., Dai, J.
- ID
- ZDB-PUB-190701-13
- Date
- 2019
- Source
- Chemical Research in Toxicology 32(7): 1432-1440 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Endocrine Disruptors/metabolism
- Endocrine Disruptors/toxicity*
- Estrogens/metabolism
- Estrogens/toxicity
- Female
- Hydrocarbons, Fluorinated/metabolism
- Hydrocarbons, Fluorinated/toxicity*
- Male
- Ovary/drug effects
- Reproduction/drug effects
- Sulfonamides/metabolism
- Sulfonamides/toxicity*
- Surface-Active Agents/metabolism
- Surface-Active Agents/toxicity*
- Testis/drug effects
- Thyroid Gland/drug effects
- Triiodothyronine/metabolism
- Vitellogenins/metabolism
- Zebrafish
- PubMed
- 31251046 Full text @ Chem. Res. Toxicol.
Citation
Shi, G., Cui, Q., Zhang, H., Cui, R., Guo, Y., Dai, J. (2019) Accumulation, Biotransformation, and Endocrine Disruption Effects of Fluorotelomer Surfactant Mixtures on Zebrafish. Chemical Research in Toxicology. 32(7):1432-1440.
Abstract
As an alternative to perfluorooctanesulfonate (PFOS), novel fluorotelomer surfactants (6:2 fluorotelomer sulfonamide alkylbetaine (6:2 FTAB) and 6:2 fluorotelomer sulfonamide alkylamine (6:2 FTAA)) are widely used in aqueous film-forming foams and are frequently found to coexist in the environment. However, their potential toxicities remain unknown. Here, we investigated the chronic toxicity of 6:2 FTAB (65%) and 6:2 FTAA (35%) coexposure on adult zebrafish at doses of 0, 5, 50, or 500 μg/L using a flow-through exposure system for 180 days. Results showed that 6:2 FTAB was undetected in adult tissue and their offspring, while 6:2 FTAA was highly dominant, accounting for ∼92% of total quantified poly/perfluoroalkyl substances (PFASs), and their metabolic products (6:2 fluorotelomer sulfonamide and 6:2 fluorotelomer sulfonate) further accounting for 2.8%-8.5%. 6:2 FTAA accumulation exhibited a sex-bias, with higher levels found in male livers than that in female, but in gonad showed an opposite pattern. Co-exposure to 6:2 FTAB and 6:2 FTAA mixture (50 and 500 μg/L) could decrease the average number of eggs production and increase the malformation and mortality in their offspring. Testosterone (T) and 17 β-estradiol (E2) levels increased in the 50 and 500 μg/L exposed females, but T level decreased in the 500 μg/L exposed males. Correspondingly, the transcriptional pattern of hypothalamus-pituitary-gonad axis genes was different between male and female. Increased liver vitellogenin levels in the 50 and 500 μg/L-exposed males indicated that these compounds might possess estrogen-like activity. Furthermore, 3,5,3'-triiodothyronine (T3) and thyroxine (T4) levels decreased in the 50 and 500 μg/L females and increased T4 level in 500 μg/L exposed males. These results suggest that 6:2 FTAB is extensively metabolized in fish, whereas 6:2 FTAB and 6:2 FTAA coexposure disrupted the adult endocrine system and impaired offspring development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping