PUBLICATION
Elevated biosynthesis of palmitic acid is required for zebrafish against Edwardsiella tarda infection
- Authors
- Xu, D., Wang, J., Guo, C., Peng, X.X., Li, H.
- ID
- ZDB-PUB-190629-5
- Date
- 2019
- Source
- Fish & shellfish immunology 92: 508-518 (Journal)
- Registered Authors
- Keywords
- Biosynthesis of unsaturated fatty acids, Edwardsiella tarda, Inactivated vaccine, Innate immunity, Metabolomics, TCA cycle
- MeSH Terms
-
- Animals
- Bacterial Vaccines
- Edwardsiella tarda/physiology
- Enterobacteriaceae Infections/immunology
- Fish Diseases/immunology
- Palmitic Acid/metabolism*
- Random Allocation
- Vaccines, Attenuated
- Vaccines, Inactivated
- Zebrafish
- PubMed
- 31247319 Full text @ Fish Shellfish Immunol.
Citation
Xu, D., Wang, J., Guo, C., Peng, X.X., Li, H. (2019) Elevated biosynthesis of palmitic acid is required for zebrafish against Edwardsiella tarda infection. Fish & shellfish immunology. 92:508-518.
Abstract
Mechanisms by which vaccines enhance immunity to combat bacterial pathogens are not fully understood. Recently, we have found that live Edwardsiella tarda vaccine enhances ability against the bacterial challenge by metabolic modulation in zebrafish. Here we first explored the metabolic modulation promoted by inactivated E. tarda to eliminate the pathogen. Inactivated E. tarda vaccine modulated a similar metabolome to combat with the pathogen in zebrafish as live E. tarda vaccine did. Specifically, both vaccines promoted biosynthesis of unsaturated fatty acids and the TCA cycle. However, due to relatively higher activated TCA cycle in inactivated vaccine than live vaccine, live vaccine promoted higher abundance of palmitate than inactivated vaccine. Consistently, the protection against E. tarda challenge was palmitate dose-dependent. Live vaccine activated higher expression of IL-1β, IL-6, Cox genes and lower expression of IL-15, IL-21 genes than inactivated vaccine, which is similar to the results stimulated by high and low doses of palmitate, respectively. These findings indicate live and inactivated E. tarda vaccines stimulate differential abundances of palmitate that contribute to differential innate immunities against bacterial infection. Thus, metabolic environment contributes to immune response.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping