PUBLICATION
Vitamin D Receptor Signaling Regulates Craniofacial Cartilage Development in Zebrafish
- Authors
- Kwon, H.J.
- ID
- ZDB-PUB-190627-2
- Date
- 2019
- Source
- Journal of developmental biology 7(2): (Journal)
- Registered Authors
- Keywords
- Vitamin D receptor (VDR), craniofacial cartilage, follistatin a (fsta), zebrafish
- MeSH Terms
- none
- PubMed
- 31234506 Full text @ J Dev Biol
Citation
Kwon, H.J. (2019) Vitamin D Receptor Signaling Regulates Craniofacial Cartilage Development in Zebrafish. Journal of developmental biology. 7(2).
Abstract
Vitamin D plays essential roles in supporting the skeletal system. The active form of vitamin D functions through the vitamin D receptor (VDR). A hereditary vitamin-D-resistant rickets with facial dysmorphism has been reported, but the involvement of VDR signaling during early stages of craniofacial development remains to be elucidated. The present study investigated whether VDR signaling is implicated in zebrafish craniofacial cartilage development using a morpholino-based knockdown approach. Two paralogous VDR genes, vdra and vdrb, have been found in zebrafish embryos. Loss-of-vdra has no discernible effect on cartilage elements, whereas loss-of-vdrb causes reduction and malformation of craniofacial cartilages. Disrupting both vdra and vdrb leads to more severe defects or complete loss of cartilage. Notably, knockdown of vdrb results in elevated expression of follistatin a (fsta), a bone morphogenetic protein (BMP) antagonist, in the adjacent pharyngeal endoderm. Taken together, these findings strongly indicate that VDR signaling is required for early craniofacial cartilage development in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping