PUBLICATION
The protective effect of fish-derived cathelicidins on bacterial infections in zebrafish, Danio rerio
- Authors
- Chen, C., Wang, A., Zhang, F., Zhang, M., Yang, H., Li, J., Su, P., Chen, Y., Yu, H., Wang, Y.
- ID
- ZDB-PUB-190617-3
- Date
- 2019
- Source
- Fish & shellfish immunology 92: 519-527 (Journal)
- Registered Authors
- Keywords
- Antimicrobial, Bacterial infection, Cathelicidin, Danio rerio, Host defense peptide, Immunomodulatory
- MeSH Terms
-
- Animals
- Cathelicidins/pharmacology*
- Fish Diseases/microbiology
- Fish Diseases/prevention & control*
- Fish Proteins/pharmacology*
- Gram-Negative Bacteria/drug effects
- Gram-Negative Bacterial Infections/prevention & control
- Gram-Negative Bacterial Infections/veterinary
- Gram-Positive Bacteria/drug effects
- Gram-Positive Bacterial Infections/prevention & control
- Gram-Positive Bacterial Infections/veterinary
- Protective Agents/pharmacology*
- Random Allocation
- Zebrafish*
- PubMed
- 31202967 Full text @ Fish Shellfish Immunol.
Citation
Chen, C., Wang, A., Zhang, F., Zhang, M., Yang, H., Li, J., Su, P., Chen, Y., Yu, H., Wang, Y. (2019) The protective effect of fish-derived cathelicidins on bacterial infections in zebrafish, Danio rerio. Fish & shellfish immunology. 92:519-527.
Abstract
Antibiotic-resistant bacteria are severe threats to aquaculture industry. Boosting and modulating host immune responses has been proved to be an effective strategy to combat with bacterial infections and there is an urgent need for novel immunomodulators. Cathelicidins is an important family of host defense peptides (HDPs) that possess direct antimicrobial activities and potent immunomodulatory properties. Several cathelicidins have been identified and characterized from diverse fish species. Considering the relatively conserved immune systems between different fish species, it is reasonable to speculate that cathelicidins from different fish species possess immunomodulating functions on the other fish species. In the present study, two fish-derived cathelicidins (CATH_BRALE and codCath1) were selected to investigate their protective effect on zebrafish with bacterial infections. They exhibited potent and broad-spectrum antimicrobial activities against the tested aquatic Gram-positive and Gram-negative pathogenic bacteria, with MIC values ranging 2.34-18.75 μg/ml for CATH_BRALE and 2.34-37.5 μg/ml for codCath1. And their antimicrobial effect is so rapid that they killed the bacteria within 60 min. Unlike conventional antibiotics, they kill bacteria by inducing bacterial membrane permeabilization and cell disruption. Besides direct antimicrobial activity, CATH_BRALE and codCath1 exhibited potent immunomodulatory functions by both inhibiting bacteria induced zebrafish pro-inflammatory cytokine gene (TNF-α, IL-1β, and IL-6) expression and stimulating zebrafish chemokine gene IL-8 expression. In vivo challenge test proved that they could significantly decrease the bacterial numbers and enhance the survival rates of zebrafish. All the results above imply the great potential of CATH_BRALE and codCath1 as novel peptide immunomodulators in fish aquaculture industry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping